Lactobacillus plantarum culture supernatants improve intestinal tissue exposed to deoxynivalenol

Q2 Medicine Experimental and Toxicologic Pathology Pub Date : 2017-10-02 DOI:10.1016/j.etp.2017.06.005

L.G. Maidana , J. Gerez , F. Pinho , S. Garcia , A.P.F.L. Bracarense

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引用次数: 13

Abstract

In the present study, histological, morphometrical and ultrastructural analysis were performed to investigate intestinal mucosa changes in piglets exposed to deoxynivalenol alone or associated with two strains of Lactobacillus plantarum and the respective culture supernatants. Jejunal explants were incubated for 4 h in culture medium with a) only culture medium (DMEM, control group), b) deoxynivalenol (DON, 10 μM), c) heat-inactivated Lactobacillus plantarum strain1 − LP1 (1.1 × 10⁸ CFU/ml) plus DON, d) heat-inactivated Lactobacillus plantarum strain2–LP2 (2.0 × 10⁹ CFU/ml) plus DON, e) heat-inactivated Lactobacillus plantarum strain1 culture supernatant (CS1) plus DON, and f) heat-inactivated Lactobacillus plantarum strain1 culture supernatant (CS1) plus DON. Explants exposed to DON and DON plus LP1 and LP2 showed a significant increase in histological changes (mainly villi atrophy and apical necrosis) and a significant decrease in villi height when compared to unexposed explants. However, explants treated with CS1 + DON and CS2 + DON remained similar to the control group both in histological and morphometrical aspects. DON also induced a significant decrease in goblet cell density compared to control whereas CS1 + DON treatment induced an increase in the number of goblet cells in comparison to DON explants. In addition, ultrastructural assessment showed control, CS1 + DON and CS2 + DON explants with well delineated finger shape villi, meanwhile DON-treated, LP1 + DON and LP2 + DON explants showed a severe villi atrophy with leukocytes exudation on the intestinal surface. Taken together, our results indicate that the culture supernatant treatment reduced the toxic effects induced by DON on intestinal tissue and may contribute as an alternative strategy to reduce mycotoxin toxicity.

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植物乳杆菌培养上清液改善暴露于脱氧雪腐镰刀菌醇的肠道组织

本研究通过组织学、形态计量学和超微结构分析，研究了仔猪单独暴露脱氧雪腐菌醇或与两株植物乳杆菌及其培养上清液联合暴露时肠道黏膜的变化。空肠外植体在a)纯培养基(DMEM，对照组)、b)脱氧腐腐菌醇(DON, 10 μM)、c)热灭活的植物乳杆菌菌株1 - LP1 (1.1 × 108 CFU/ml)加DON、d)热灭活的植物乳杆菌菌株2 - lp2 (2.0 × 109 CFU/ml)加DON、e)热灭活的植物乳杆菌菌株1培养上清(CS1)加DON、f)热灭活的植物乳杆菌菌株1培养上清(CS1)加DON的培养基中培养4 h。与未暴露的外植体相比，暴露于DON和DON + LP1和LP2的外植体的组织学变化(主要是绒毛萎缩和根尖坏死)显著增加，绒毛高度显著降低。然而，CS1 + DON和CS2 + DON处理的外植体在组织学和形态计量学方面与对照组相似。与对照组相比，DON还诱导了杯状细胞密度的显著降低，而CS1 + DON处理则诱导了杯状细胞数量的增加。超微结构观察显示，对照组CS1 + DON和CS2 + DON外植体具有清晰的指状绒毛，而经DON处理的LP1 + DON和LP2 + DON外植体肠表面出现了严重的绒毛萎缩和白细胞渗出。综上所述，我们的研究结果表明，培养上清处理降低了DON对肠道组织的毒性作用，可能有助于降低霉菌毒素毒性的替代策略。

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来源期刊

Experimental and Toxicologic Pathology 医学-病理学

CiteScore

2.08

自引率

0.00%

发文量

审稿时长

5.3 weeks

期刊介绍： Cessation. The international multidisciplinary journal is devoted to the publication of studies covering the whole range of experimental research on disease processes and toxicology including cell biological investigations. Its aim is to support progress in the interdisciplinary cooperation of researchers working in pathobiology, toxicology, and cell biology independent of the methods applied. During the past decades increasing attention has been paid to the importance of toxic influence in the pathogenesis of human and animal diseases. This is why Experimental and Toxicologic Pathology meets the urgent need for an interdisciplinary journal felt by a wide variety of experts in medicine and biology, including pathologists, toxicologists, biologists, physicians, veterinary surgeons, pharmacists, and pharmacologists working in academic, industrial or clinical institutions.