Antimicrobial Activity of Cultivable Endophytic Fungi Associated with Hancornia Speciosa Gomes Bark.

Q3 Immunology and Microbiology Open Microbiology Journal Pub Date : 2017-09-21 eCollection Date: 2017-01-01 DOI:10.2174/1874285801711010179

Mardonny Bruno de Oliveira Chagas, Irailton Prazeres Dos Santos, Luis Claudio Nascimento da Silva, Maria Tereza Dos Santos Correia, Janete Magali de Araújo, Marilene da Silva Cavalcanti, Vera Lucia de Menezes Lima

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引用次数: 31

Abstract

Introduction: In this study, we evaluated the antimicrobial potential of cultivable endophytic fungi associated with Hancornia speciosa Gomes stem bark.

Methods and materials: Plant samples were collected in rainy (July 2010) and dry (January 2011) seasons. In total, 116 endophytic fungi strains were isolated from 90 fragments (64.4% frequency of colonization). Higher fungi frequency was observed in the rainy season (84.4%). The strains were grouped into 14 species; the most frequent were Phoma cava (13.8%), Colletotrichum gloeosporioides (12.1%), and Lasiodiplodia theobromae (11.2%). Fungal diversity was similar in both the seasons. Among the 116 strains, 39 (33.6%) showed antimicrobial activity in preliminary screening. The ten most active isolates were subjected to semi-solid fermentation using rice or corn as substrates. Methanolic extracts were obtained from each fermentation medium and the minimum inhibitory (MIC) and minimum microbicide concentrations (MMC) were determined.

Results: The best antimicrobial results (MIC < 100 µg/mL) were observed for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 µg/mL) and Staphylococcus aureus (MIC = 39 µg/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 µg/mL).

Conclusion: Thus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.

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山茱萸树皮内生真菌抑菌活性研究。

摘要:本研究对汉草茎皮内生真菌的抑菌潜力进行了研究。方法与材料:植物标本采集时间为雨季(2010年7月)和旱季(2011年1月)。90个片段共分离到116株内生真菌，定殖率为64.4%。真菌出现频率在雨季较高(84.4%)。菌株分为14种;最常见的分别为:cava(13.8%)、gloeosporioides(12.1%)、Lasiodiplodia可可(11.2%)。真菌多样性在两个季节相似。初步筛选116株病原菌中有39株(33.6%)具有抗菌活性。以水稻或玉米为底物进行半固态发酵。从每种发酵培养基中提取甲醇提取物，并测定最小抑菌浓度(MIC)和最小杀微生物浓度(MMC)。结果:在水稻培养基中培养的真菌菌株:黑曲霉FHS061对神奇变形杆菌(MIC = 19µg/mL)和金黄色葡萄球菌(MIC = 39µg/mL)的抑菌效果最好(MIC < 100µg/mL)。这些菌株在玉米培养基(MIC = 78µg/mL)中也表现出良好的抗mirabilis效果。结论:槐茎皮中含有多种具有抗菌潜力的内生真菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.