Effects of CAR-regimen autohemochemotherapy on redox-dependent processes in erythrocytes of tumor-bearing organism in experimental ovarian cancer.

T P Gening, D R Dolgova, T V Abakumova, I I Antoneeva, E Yu Nasyrova, S O Gening
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Abstract

The purpose: The purpose of the study was to investigate the effect of the scheme autohemochemotherapy ATS on redox-dependent processes in red blood cells of tumor-bearing organism at an experimental ovarian cancer.

Methods: We studied the dynamics of redox-dependent processes in red blood cells of rats with experimental ascitic ovarian tumor during CAP-regimen autohemochemotherapy (cyclophosphamide, doxorubicin and cisplatin). We assessed the indicators of oxidative modification of proteins in erythrocytes - carbonyl derivatives at l = 346 nm, 370 nm, 430 nm and 530 nm, the parameters of lipid peroxidation - malondialdehyde, ketodienes, diene conjugates, Schiff bases; the enzymatic part of antioxidant system - the activity of catalase, glutathione transferase and superoxide dismutase biochemically.

Results: The red blood cells of tumor-bearing animals were found having increased the products of lipid peroxidation and oxidative modification of proteins while reducing the activity of antioxidant enzymes, suggesting a state of oxidative and carbonyl stress.

Conclusion: We showed that extracorporeal incubation of cytotoxic drugs used in the CAP scheme with autoblood prior to infusion - the method of autohemochemotherapy - either in monochemotherapy, or in CAP-regimen, decreases the levels of lipid peroxidation, oxidative modification of proteins and increases activity of first line antioxidant defense enzymes - catalase and superoxide dismutase in circulating red blood cells. Such dynamics of redox-dependent processes suggests a stabilizing effect of autohemochemotherapy on circulating erythrocytes in a tumor-bearing organism.

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car方案自体血液化疗对实验性卵巢癌荷瘤机体红细胞氧化还原依赖过程的影响。
目的:本研究的目的是探讨自体血液化疗方案ATS对实验性卵巢癌荷瘤细胞氧化还原依赖过程的影响。方法:研究cap方案自体血液化疗(环磷酰胺、阿霉素和顺铂)对实验性卵巢腹水性肿瘤大鼠红细胞氧化还原依赖过程的影响。我们评估了红细胞中蛋白质氧化修饰的指标-羰基衍生物在l = 346 nm, 370 nm, 430 nm和530 nm,脂质过氧化参数-丙二醛,酮二烯,二烯偶联物,希夫碱;抗氧化系统的酶类部分-过氧化氢酶、谷胱甘肽转移酶和超氧化物歧化酶的生物化学活性。结果:荷瘤动物红细胞中脂质过氧化产物和蛋白质氧化修饰增加,抗氧化酶活性降低,提示其处于氧化和羰基应激状态。结论:我们发现,无论是单药化疗还是单药化疗,CAP方案中使用的细胞毒性药物在输注前与自身血液进行体外培养,都能降低循环红细胞的脂质过氧化、蛋白质的氧化修饰水平,并增加一线抗氧化防御酶-过氧化氢酶和超氧化物歧化酶的活性。这种氧化还原依赖过程的动力学表明,自身血液化疗对荷瘤生物体内循环红细胞具有稳定作用。
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