[SSR polymorphism of modern cultivars and autochthonous forms of the pear tree from North Caucasus].

Genetika Pub Date : 2016-11-01
I I Suprun, S V Tokmakov, I A Bandurko, E T Ilnitskaya
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Abstract

Genetic similarity and relatedness within the set of pear genotypes including autochthonous Circassian cultivars from North Caucasus, European cultivars, accessions of Pyrus caucasica Fed., and modern Russian cultivars were estimated on the basis of analysis of SSR loci. The level of polymorphism for the studied loci varied from 11 to 15 alleles per locus in the set of 29 samples of pears. A higher level of allelic polymorphism of SSR loci was revealed for a set of P. caucasica samples in comparison with modern cultivated cultivars: from 9 to 12 alleles for P. caucasica and from 6 to 8 alleles for modern cultivars. Specific alleles for the mentioned groups of pears were identified. UPGMA clustering revealed two distinct groups: one includes P. caucasica accessions and autochthonous Caucasian cultivars and the other group includes all cultivated European and Russian pear cultivar. The results support the hypothesis of an isolated gene pool formation of autochthonous pear cultivars of the North Caucasus and their probable origin from the wild P. caucasica.

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[北高加索地区梨树现代品种和乡土品种的SSR多态性]。
通过SSR位点分析,估计了北高加索地区切尔克斯梨品种、欧洲品种、高加索梨新品种和现代俄罗斯品种间的遗传相似性和亲缘性。在29个梨样本中,每个位点的多态性水平在11 ~ 15个等位基因之间。与现代栽培品种相比,一组白种人样品的SSR位点多态性较高,等位基因在9 ~ 12个之间,现代栽培品种在6 ~ 8个之间。鉴定了上述梨群的特异等位基因。UPGMA聚类显示两个不同的群体:一个群体包括高加索梨和本土高加索品种,另一个群体包括所有栽培的欧洲和俄罗斯梨品种。这一结果支持了北高加索地区梨品种形成孤立基因库的假设,以及它们可能起源于野生高加索梨的假设。
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