Optimizing T Cell Expansion in a Hollow-Fiber Bioreactor.

IF 2.3 Q4 CELL & TISSUE ENGINEERING Current Stem Cell Reports Pub Date : 2018-01-01 Epub Date: 2018-02-27 DOI:10.1007/s40778-018-0116-x

Brian Nankervis, Mark Jones, Boah Vang, R Brent Rice, Claire Coeshott, Jim Beltzer

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引用次数: 24

Abstract

Purpose of review: Recent developments in regenerative medicine have precipitated the need to expand gene-modified human T cells to numbers that exceed the capacity of well-plate-based, and flask-based processes. This review discusses the changes in process development that are needed to meet the cell expansion requirements by utilizing hollow-fiber bioreactors. Maintenance of cell proliferation over long periods can become limited by unfilled demands for nutrients and oxygen and by the accumulation of waste products in the local environment.

Recent findings: Perfusion feeding, improved gas exchange, and the efficient removal of lactate can increase the yield of T cells from an average of 10.8E +09 to more than 28E +09 in only 10 days.

Summary: Aggressively feeding cells and actively keeping cells in the bioreactor improves gas exchange and metabolite management over semi-static methods. The ability to remove the environmental constraints that can limit cell expansion by using a two-chamber hollow-fiber bioreactor will be discussed.

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在中空纤维生物反应器中优化T细胞扩增。

综述目的:再生医学的最新发展促使人们需要扩大基因修饰的人类T细胞的数量，以超过基于孔板和基于烧瓶的方法的能力。本文综述了利用中空纤维生物反应器满足细胞扩增要求所需的工艺发展变化。由于对营养物质和氧气的需求未得到满足，以及当地环境中废物的积累，细胞增殖的长期维持可能受到限制。最近的研究发现:灌注饲喂、改善气体交换和有效去除乳酸可以在短短10天内将T细胞产量从平均10.8E +09提高到28E +09以上。与半静态方法相比，积极喂养细胞和积极保持细胞在生物反应器中可以改善气体交换和代谢物管理。将讨论利用双腔中空纤维生物反应器消除环境限制的能力。

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来源期刊

Current Stem Cell Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

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0.00%

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期刊介绍： The goal of this journal is to publish cutting-edge reviews on subjects pertinent to all aspects of stem cell research, therapy, ethics, commercialization, and policy. We aim to provide incisive, insightful, and balanced contributions from leading experts in each relevant domain that will be of immediate interest to a wide readership of clinicians, basic scientists, and translational investigators. We accomplish this aim by appointing major authorities to serve as Section Editors in key subject areas across the discipline. Section Editors select topics to be reviewed by leading experts who emphasize recent developments and highlight important papers published over the past year on their topics, in a crisp and readable format. We also provide commentaries from well-known figures in the field, and an Editorial Board of internationally diverse members suggests topics of special interest to their country/region and ensures that topics are current and include emerging research.