Does Dietary Provision of Guanidinoacetic Acid Induce Global DNA Hypomethylation in Healthy Men and Women?

IF 2 4区医学 Q3 GENETICS & HEREDITY Lifestyle Genomics Pub Date : 2018-01-01 Epub Date: 2018-04-03 DOI:10.1159/000487336

Sergej M Ostojic, Marija Mojsin, Patrik Drid, Milan Vranes

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引用次数: 3

Abstract

Background/aims: Guanidinoacetic acid (GAA) is an experimental dietary additive and has been reported to induce methyl depletion when provided by the diet. However, no study evaluated whether supplemental GAA affects DNA methylation, a critical epigenetic process for genome regulation.

Methods: In this open-label, repeated-measure interventional trial, we evaluated the impact of 12 weeks of GAA supplementation on global DNA methylation in 14 healthy participants (8 women and 6 men, age 22.2 ± 2.3 years, body mass index 24.8 ± 5.7).

Results: Dietary provision of GAA had no effect on global DNA methylation, with 5-methylcytosine (m5C) nonsignificantly increased by 13.4% at postadministration when averaged across participants (95% confidence interval -5.5 to 32.3; p = 0.26). Notable DNA hypomethylation (corresponding to a 5% drop in m5C) was found in 3 of 14 participants at follow-up.

Conclusion: Global DNA methylation seems to be unaltered by dietary provision of 3 g of GAA per day for 12 weeks in healthy men and women.

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膳食中添加胍基乙酸是否会诱导健康男性和女性整体DNA低甲基化?

背景/目的:胍基乙酸(GAA)是一种试验性日粮添加剂，据报道，在日粮中添加GAA会导致甲基消耗。然而，没有研究评估补充GAA是否会影响DNA甲基化，这是基因组调控的关键表观遗传过程。方法:在这项开放标签、重复测量的干介性试验中，我们评估了14名健康参与者(8名女性和6名男性，年龄22.2±2.3岁，体重指数24.8±5.7)补充12周GAA对整体DNA甲基化的影响。结果:饮食中添加GAA对整体DNA甲基化没有影响，5-甲基胞嘧啶(m5C)在给药后增加了13.4%(95%置信区间为-5.5至32.3;P = 0.26)。在随访中，14名参与者中有3人发现了显著的DNA低甲基化(相当于m5C下降5%)。结论:在健康男性和女性中，连续12周每天摄入3g GAA似乎不会改变整体DNA甲基化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lifestyle Genomics Agricultural and Biological Sciences-Food Science

CiteScore

4.00

自引率

7.70%

发文量

审稿时长

28 weeks

期刊介绍： Lifestyle Genomics aims to provide a forum for highlighting new advances in the broad area of lifestyle-gene interactions and their influence on health and disease. The journal welcomes novel contributions that investigate how genetics may influence a person’s response to lifestyle factors, such as diet and nutrition, natural health products, physical activity, and sleep, amongst others. Additionally, contributions examining how lifestyle factors influence the expression/abundance of genes, proteins and metabolites in cell and animal models as well as in humans are also of interest. The journal will publish high-quality original research papers, brief research communications, reviews outlining timely advances in the field, and brief research methods pertaining to lifestyle genomics. It will also include a unique section under the heading “Market Place” presenting articles of companies active in the area of lifestyle genomics. Research articles will undergo rigorous scientific as well as statistical/bioinformatic review to ensure excellence.