[Progress in microbial single-cell genomics for environmental microbiology research - A review].

微生物学报 Pub Date : 2016-11-04
Yi Wang, Peng Xu, Xin Dai
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引用次数: 0

Abstract

We compared different methods used in single-cell isolation and whole genome amplification of microbial cells, and summarized research progresses in single-cell genomics (SCG) in studying environmental microbes. The studies of single cell and SCG have become one of the hot topics in life sciences in recent years. As a newly developed technology following microbial metagenomics, microbial SCG can efficiently explore large amounts of information of the uncultivable microbial genomics from environment. Generally, following steps need to be taken to obtain microbial SCG:isolation of single microbial cells, whole genome amplification of the single cells, amplicon sequencing, and data analysis. Microbial SCG can be widely used to explore new functional genes that are not detectable by metagenomics and other traditional methods, detect uncultured microbes with extremely low abundance, and study the life evolution of microbial cells.

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环境微生物单细胞基因组学研究进展综述
比较了微生物细胞单细胞分离和全基因组扩增的不同方法,综述了单细胞基因组学在环境微生物研究中的研究进展。近年来,单细胞和细胞间质细胞的研究已成为生命科学研究的热点之一。微生物SCG是继微生物宏基因组学之后发展起来的一项新技术,它可以有效地从环境中挖掘出大量不可培养的微生物基因组学信息。通常,获得微生物SCG需要采取以下步骤:单个微生物细胞的分离,单个细胞的全基因组扩增,扩增子测序和数据分析。微生物SCG可广泛用于探索元基因组学等传统方法无法检测到的新功能基因,检测丰度极低的未培养微生物,研究微生物细胞的生命进化。
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来源期刊
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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