{"title":"[Construction of SPA7074-deficient mutant of biocontrol strain Streptomyces pactum Act12 and characterization of its secondary metabolites].","authors":"Xuemei Duan, Feiyang Zhao, Xia Yan, Quanhong Xue, Xiaoxia Li, Bingjie Wen, Lianghui Jia, Hua Yan","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To disrupt spa7074, which encodes a member of the TetR family transcriptional factors, in biocontrol strain Act12 and characterize the secondary metabolites in the mutant strain.</p><p><strong>Methods: </strong>We disrupted the gene spa7074 by homologous recombination. The secondary metabolites of the mutant strain Δspa7074 and Act12 were detected by HPLC. The structure was analyzed by MS and NMR.</p><p><strong>Results: </strong>Compared to the wild-type strain, the production of some unknown compounds in the mutant strain Δspa7074 increased obviously. We purified one of the compounds and identified as oligomycin D by MS and NMR analysis.</p><p><strong>Conclusion: </strong>An oligomycin D-producing strain Δspa7074 was derived via genetic engineering.</p>","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"56 12","pages":"1883-91"},"PeriodicalIF":0.0000,"publicationDate":"2016-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"微生物学报","FirstCategoryId":"1089","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To disrupt spa7074, which encodes a member of the TetR family transcriptional factors, in biocontrol strain Act12 and characterize the secondary metabolites in the mutant strain.
Methods: We disrupted the gene spa7074 by homologous recombination. The secondary metabolites of the mutant strain Δspa7074 and Act12 were detected by HPLC. The structure was analyzed by MS and NMR.
Results: Compared to the wild-type strain, the production of some unknown compounds in the mutant strain Δspa7074 increased obviously. We purified one of the compounds and identified as oligomycin D by MS and NMR analysis.
Conclusion: An oligomycin D-producing strain Δspa7074 was derived via genetic engineering.
期刊介绍：
Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal
publishes original papers,reviews in microbiological science,and short communications describing unusual observations.
Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database
(CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical
Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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