[Metabolic pattern of pig hindgut bacteria on aromatic amino acids by an in vitro fermentation method].

微生物学报 Pub Date : 2016-11-04
Meilei Ma, Xiangyu He, Weiyun Zhu
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引用次数: 0

Abstract

Objective: This experiment was conducted to study different metabolic patterns of pig hindgut bacteria on aromatic amino acids by an in vitro fermentation method.

Methods: Ileum, cecum and colon chyme in Duroc, Landrace and Yorkshire goods hybridization pigs were taken as inoculum. The single aromatic amino acid concentration was kept 10 mmol/L in fermentation flask. Then the fermentation flask was incubated at 37℃ for 24 h. Gas production was measured at 4, 8, 12, 16 and 24 h, and samples of fermentation collected at 0 h and 24 h were used to measure ammonia nitrogen NH3-N and microbial crude protein (MCP). Denaturing gradient gel electrophoresis (DGGE) and real-time PCR were used to monitor and quantify the development of bacteria community in zymotic fluid.[

Results: The concentrations of NH3-N and MCP were significantly affected by aromatic amino acids and intestinal segments (P<0.01). Intestinal segments also affected gas production (GP) significantly (P0.01). NH3-N, MCP and GP were affected by interaction of aromatic amino acids and intestinal segments. DGGE analysis showed bacteria of aromatic amino acids shared amount of bands together, especially similarity analysis of DGGE profile of Phe and Tyr in ileum, Tyr and Trp in colon were 87.9% and 80.5% separately. Shannon diversity indices analysis revealed that aromatic amino acids in cecum and colon varied significantly (P<0.05). Real-time PCR results showed that the quantity of total bacteria were affected by aromatic amino acids and intestinal segments significantly (P<0.05).

Conclusion: The potential as proportion of different aromatic amino acids are different. Compared with Trp and Phe, the diversity of bacteria utilizing Tyr in cecum or colon is low; compared with Tyr and Trp, a large number of Phe participated in synthesizing bacteria.The fermentation pattern of specific aromatic amino acids in different intestinal segment was unique. Compared with ileum and cecum, much more aromatic amino acids participated in the synthesis of bacteria in colon.

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[猪后肠细菌体外发酵对芳香氨基酸的代谢模式]。
目的:采用体外发酵法研究猪后肠细菌对芳香氨基酸的代谢模式。方法:以杜洛克猪、长白猪和约克郡猪的回肠、盲肠和结肠食糜为接种物。发酵瓶中单芳香氨基酸浓度保持在10 mmol/L。37℃恒温培养24 h,测定4、8、12、16、24 h产气量,0、24 h收集的发酵样品测定氨氮NH3-N和微生物粗蛋白(MCP)。采用变性梯度凝胶电泳(DGGE)和实时荧光定量PCR技术对酵母液中细菌群落的发育进行监测和定量。[结果]NH3-N和MCP浓度受芳香族氨基酸和肠段的影响显著(p)结论:不同芳香族氨基酸的电位占比不同。与色氨酸和苯丙氨酸相比,盲肠和结肠中利用酪氨酸的细菌多样性较低;与Tyr和Trp相比,大量的Phe参与了细菌的合成。特定芳香氨基酸在不同肠段的发酵模式是独特的。与回肠和盲肠相比,结肠中参与细菌合成的芳香氨基酸要多得多。
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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