[A novel phylogenetic lineage clustered by NIa gene of Sugarcane streak mosaic virus Yunnan isolates].

微生物学报 Pub Date : 2016-11-04
Zhen He, Wenfeng Li, Shifang Li
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引用次数: 0

Abstract

Objective: We assessed the phylogenetic relationship of Sugarcane streak mosaic virus (SCSMV) according to NIa sequences, to infer the prevalence and variation of SCSMV and to prevent and control this virus.

Methods: Leaf samples with mosaic symptom were collected from sugarcane-growing areas in Yunnan province and the Chinese national nursery of sugarcane germplasm resources (NNSGR). NIa sequences of SCSMV were determined by RT-PCR, and analyzed by Splits Tree, RDP, PhyML and DnaSP softwares, in aspect of phylogenetic, selection, and gene flow.

Results: We obtained 23 NIa sequences; clear recombination site was not found in NIa; a novel cluster formed by SCSMV Yunnan isolates determined here was found; strong purifying selection was found in NIa of SCSMV; and the gene flow of SCSMV subpopulations between sugarcane-growing areas in Yunnan province and the NNSGR was not frequent.

Conclusion: Similar with P1, HC-Pro and CP genes, SCSMV isolates could be divided into five clusters. NIa of SCSMV Yunnan isolates showed high genetic diversity and clear geographical distribution.

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甘蔗条纹花叶病毒云南分离株NIa基因聚类的新系统发育谱系。
目的:根据NIa序列评价甘蔗条纹花叶病毒(SCSMV)的系统发育关系,推断SCSMV的流行和变异,为防治该病毒提供依据。方法:采集云南省甘蔗产区和国家甘蔗种质资源苗圃的花叶病叶片样本。采用RT-PCR方法确定SCSMV的NIa序列,并利用splitting Tree、RDP、PhyML和DnaSP软件进行系统发育、选择和基因流分析。结果:获得23条NIa序列;NIa中未发现明显的重组位点;发现云南SCSMV分离株形成一个新的集群;SCSMV的NIa存在较强的纯化选择;云南甘蔗产区与NNSGR间SCSMV亚群的基因流动不频繁。结论:SCSMV分离株与P1、HC-Pro和CP基因相似,可分为5个聚类。云南分离株遗传多样性高,地理分布清晰。
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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