[Changes of microbial abundance and functional genes in oil and gas under gaseous hydrocarbon condition].

微生物学报 Pub Date : 2017-01-04
Lei Gu, Ze Mei, Kewei Xu, Yuping Tang, Fan Yang, Yongge Sun
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引用次数: 0

Abstract

Objective: Hydrocarbon microseepage is a natural phenomenon that hydrocarbon gases of subsurface petroleum accumulations migrate upward by reservoir pressure. The detection of the activity and distribution of these highly specialized populations can be used to forecast the existence of oil and gas deposits. However, the hydrocarbon-oxidizing bacterial population are usually not predominant in soil samples above the typical onshore oil and gas reservoirs. It is hard to assess the abundance of hydrocarbon-oxidizing bacteria.

Methods: In this study, changes of microbial abundance and functional genes were studied.

Results: Under gaseous hydrocarbon condition, changes of methane and butane oxidizing bacteria were different. Furthermore, changes of functional genes indicated that genome analysis was more proper for microbial anomalies detection.

Conclusion: The profiling data of this study provide a comprehensive insight into gene expression profiles and lay the foundation for optimizing the microbial prospecting technology.

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[气态烃条件下油气微生物丰度及功能基因的变化]。
目的:烃类微渗流是地下油气藏烃类气体受储层压力向上运移的一种自然现象。探测这些高度专业化的种群的活动和分布可以用来预测石油和天然气矿床的存在。然而,在典型的陆上油气储层以上的土壤样品中,烃类氧化细菌种群通常并不占优势。很难评估碳氢化合物氧化细菌的丰度。方法:研究微生物丰度和功能基因的变化。结果:气态烃条件下,甲烷氧化菌和丁烷氧化菌的变化不同。此外,功能基因的变化表明基因组分析更适合微生物异常检测。结论:本研究的分析数据为全面了解基因表达谱提供了依据,为优化微生物找矿技术奠定了基础。
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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