[Complete genome sequencing of polymalic acid-producing strain Aureobasidium pullulans CCTCC M2012223].

微生物学报 Pub Date : 2017-01-04
Yongkang Wang, Xiaodan Song, Xiaorong Li, Sang-tian Yang, Xiang Zou
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Abstract

Objective: To explore the genome sequence of Aureobasidium pullulans CCTCC M2012223, analyze the key genes related to the biosynthesis of important metabolites, and provide genetic background for metabolic engineering.

Methods: Complete genome of A. pullulans CCTCC M2012223 was sequenced by Illumina HiSeq high throughput sequencing platform. Then, fragment assembly, gene prediction, functional annotation, and GO/COG cluster were analyzed in comparison with those of other five A. pullulans varieties.

Results: The complete genome sequence of A. pullulans CCTCC M2012223 was 30756831 bp with an average GC content of 47.49%, and 9452 genes were successfully predicted. Genome-wide analysis showed that A. pullulans CCTCC M2012223 had the biggest genome assembly size. Protein sequences involved in the pullulan and polymalic acid pathway were highly conservative in all of six A. pullulans varieties. Although both A. pullulans CCTCC M2012223 and A. pullulans var. melanogenum have a close affinity, some point mutation and inserts were occurred in protein sequences involved in melanin biosynthesis.

Conclusion: Genome information of A. pullulans CCTCC M2012223 was annotated and genes involved in melanin, pullulan and polymalic acid pathway were compared, which would provide a theoretical basis for genetic modification of metabolic pathway in A. pullulans.

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[产聚苹果酸菌株普鲁兰金黄色葡萄球菌CCTCC M2012223全基因组测序]。
目的:探索普鲁兰Aureobasidium pululans CCTCC M2012223的基因组序列,分析其重要代谢物生物合成相关的关键基因,为代谢工程提供遗传背景。方法:采用Illumina HiSeq高通量测序平台对芦兰CCTCC M2012223全基因组进行测序。然后,与其他5个普鲁兰品种进行片段组装、基因预测、功能注释和GO/COG聚类分析。结果:pululans CCTCC M2012223全基因组序列为30756831 bp,平均GC含量为47.49%,成功预测9452个基因。全基因组分析显示,普鲁兰芽孢杆菌CCTCC M2012223基因组组装大小最大。普鲁兰和聚苹果酸途径相关的蛋白序列在6个普鲁兰品种中均高度保守。虽然a . pululans CCTCC M2012223与a . pululans var. melanogenum亲缘关系密切,但在黑色素生物合成相关的蛋白序列中出现了一些点突变和插入。结论:对普鲁兰CCTCC M2012223基因组信息进行标注,并对黑色素、普鲁兰和聚苹果酸途径相关基因进行比较,为普鲁兰代谢途径的基因改造提供理论依据。
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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