[Regulation of sporulation by two-component system YvcPQ in Bacillus thuringiensis].

微生物学报 Pub Date : 2017-01-04
Qingyun Fan, Shumeng Zhang, Yujing Gong, Jin He
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引用次数: 0

Abstract

Objective: To study the regulation of sporulation controlled by two-component system (TCS) YvcPQ.

Methods: β-galactosidase experiment was used to verify the regulation of YvcP on kapD expression; bacterial one-hybrid assay, EMSA and RT-qPCR were applied to study the regulation of AbrB on yvcPQ expression; markerless gene deletion coupled with spore count was used to reveal the influence of yvcPQ and kapD expressions on sporulation.

Results: transcriptional regulator AbrB up-regulated the expression of yvcPQ; YvcP promoted the expression of kapD to inhibit sporulation.

Conclusion: AbrB up-regulated the transcription of yvcPQ operon, then the increased YvcP strengthened the transcriptional acitivation of sporulation inhibitor gene kapD, and subsequently inhibited sporulation.

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[双组分体系YvcPQ对苏云金芽孢杆菌产孢的调控]。
目的:研究双组分系统(TCS) YvcPQ对产孢的调控作用。方法:采用β-半乳糖苷酶实验验证YvcP对kapD表达的调控作用;采用细菌单杂交法、EMSA法和RT-qPCR法研究AbrB对yvcPQ表达的调控;利用无标记基因缺失和孢子数结合的方法,揭示了yvcPQ和kapD表达对孢子形成的影响。结果:转录调控因子AbrB上调yvcPQ的表达;YvcP促进kapD表达抑制产孢。结论:AbrB上调了yvcPQ操纵子的转录,YvcP的升高增强了产孢抑制基因kapD的转录激活,从而抑制了产孢。
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期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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