[Chemotaxis and characteristics of chemotactic genes in Novosphingobium strains].

微生物学报 Pub Date : 2017-03-04

Xiaojing Liao, Luxi Yang, Hetharua Buce, Mengkai Zhou, Tianling Zheng, Yun Tian

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Abstract

Objective: The present study aims to analyze the chemotaxis genes and proteins of several PAH-degrading Novosphingobium strains, and the chemotaxis of these strains toward aromatic compounds and intermediates.

Methods: Based on genome comparative analysis, we identified the chemotaxis genes organization and proteins distribution. We used drop and swarm plate assays to detect the chemotaxis of these strains toward aromatic compounds and intermediates of TCA cycle.

Results: We found that all these Novosphingobium strains showed chemotaxis, but the chemotatic ability varied. The completed genome sequenced strains N. pentaromativorans F2, N. pentaromativorans US6-1, N. pentaromativorans PP1Y, Novosphingobium sp. AP12, Novosphingobium sp. Rr 2-17, and Novosphingobium nitrogenifigens DSM 19370 contained MCP, CheW, CheA, CheB, CheR and CheY. Strain F2, US6-1 and PP1Y, shared a consistent order of chemotaxis genes in "che" cluster. The chemotatic system of these Novosphingobium strains belonged to the Fla chemotactic system.

Conclusion: These strains all contained a complete chmotaxis pathway. Their chemotactic ability toward aromatic compounds and intermediates varied, and the chemotaxis of US6-1 was obvious.

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[Novosphingobium菌株趋化性及趋化基因特征]。

目的:分析几种降解多环芳烃的Novosphingobium菌株的趋化基因和趋化蛋白，以及这些菌株对芳香族化合物和中间体的趋化性。方法:通过基因组比对分析，鉴定趋化基因的组织和蛋白分布。采用滴法和群体平板法检测了这些菌株对芳香族化合物和TCA循环中间体的趋化性。结果:各菌株均具有趋化性，但趋化能力各不相同。完成基因组测序的菌株N. pentaromativorans F2、N. pentaromativorans US6-1、N. pentaromativorans PP1Y、Novosphingobium sp. AP12、Novosphingobium sp. Rr 2-17和Novosphingobium nitrogen - gengens DSM 19370含有MCP、CheW、CheA、CheB、CheR和CheY。菌株F2, US6-1和PP1Y在“che”集群中具有一致的趋化基因顺序。这些新藻属菌株的趋化系统属于Fla趋化系统。结论:这些菌株均具有完整的趋同途径。它们对芳香族化合物和中间体的趋化能力各不相同，US6-1的趋化性较明显。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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