[Phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains isolated from different geographical locations].

微生物学报 Pub Date : 2017-04-04

Pengwu Lin, Minrui Liu, Min Zhang, Aiying Wang, Yongqing Ni

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Abstract

Objective: To study the phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains from various geographical locations, as well as the relationship between the DNA fingerprinting classification and geographical origin of Acidithiobacillus.

Methods: Partial 16S-23S rRNA gene intergenic spacer (ITS) was used to construct corresponding phylogenetic trees based on the sequence homology. rus gene amplification and rep-PCR assay with two different primers (BOXAIR and ERIC) were performed to analyze genetic heterogeneity of Acidithiobacillus strains from diverse environment.

Results: Acidithiobacillus revealed a great genetic heterogeneity. The whole isolates were classified into five groups by ITS sequence analysis. This result was similar with that obtained by rep-PCR. Acidithiobacillus ferrooxidans strains were always divided into two groups of phylogenetic and BOXAIR fingerprinting cluster analysis. However, these were clustered one group in the ERIC dendrogram. Genotypic analysis of the rus gene suggested that different iron oxidation pathways have been evolved in these closely related bacteria. Taken together, the iron oxidation pathway of Acidithiobacillus and phylogenetic groups have no obvious correlation. ITS gene has been proven very useful in distinguishing closely related species or subspecies of Acidithiobacillus, to BOXAIR-PCR, which has been recommended as reliable tool for genetic heterogeneity analysis of Acidithiobacillus.

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[不同地理位置分离的23株酸性硫杆菌的系统发育和遗传异质性]。

目的:研究不同地理位置的23株酸性硫杆菌的系统发育和遗传异质性，以及DNA指纹图谱分类与酸性硫杆菌地理来源的关系。方法:采用部分16S-23S rRNA基因间间隔(ITS)基于序列同源性构建相应的系统发育树。采用两种不同引物(BOXAIR和ERIC)对不同环境下的酸性硫杆菌进行基因扩增和rep-PCR分析。结果:酸性硫杆菌具有很强的遗传异质性。通过ITS序列分析将整个菌株分为5个类群。该结果与rep-PCR结果相似。酸性氧化亚铁硫杆菌的系统发育和BOXAIR指纹聚类分析均将菌株分为两类。然而，这些都聚集在ERIC树形图中的一个组。rus基因的基因型分析表明，这些密切相关的细菌已经进化出不同的铁氧化途径。综上所述，酸性硫杆菌的铁氧化途径与系统发育类群无明显相关性。ITS基因已被证明对酸性硫杆菌的近缘种或亚种的区分非常有用，BOXAIR-PCR已被推荐为酸性硫杆菌遗传异质性分析的可靠工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

微生物学报

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7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.

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