Optimization of Xylanase Production from Aspergillus foetidus in Soybean Residue.

Q2 Biochemistry, Genetics and Molecular Biology Enzyme Research Pub Date : 2018-04-11 eCollection Date: 2018-01-01 DOI:10.1155/2018/6597017
Luana Cunha, Raquel Martarello, Paula Monteiro de Souza, Marcela Medeiros de Freitas, Kleber Vanio Gomes Barros, Edivaldo Ximenes Ferreira Filho, Mauricio Homem-de-Mello, Pérola Oliveira Magalhães
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引用次数: 37

Abstract

Enzymatic hydrolysis is an important but expensive step in the process to obtain enzyme derived products. Thus, the production of efficient enzymes is of great interest for this biotechnological application. The production of xylanase by Aspergillus foetidus in soybean residues was optimized using 2 × 23 factorial designs. The experimental data was fitted into a polynomial model for xylanase activity. Statistical analyses of the results showed that variables pH and the interaction of pH and temperature had influenced the production of xylanase, with the best xylanase production level (13.98 U/mL) occurring at fermentation for 168 hours, pH 7.0, 28°C, and 120 rpm.

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大豆渣中胎儿曲霉产木聚糖酶的优化研究。
酶水解是获得酶衍生产物过程中一个重要但昂贵的步骤。因此,高效酶的生产对这一生物技术应用具有重要意义。采用2 × 23因子设计优化了胎儿曲霉在大豆渣中生产木聚糖酶的条件。实验数据拟合为木聚糖酶活性的多项式模型。统计分析结果表明,pH变量以及pH和温度的相互作用影响了木聚糖酶的产量,在发酵168小时、pH 7.0、28°C、120 rpm时,木聚糖酶的产量最高(13.98 U/mL)。
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Enzyme Research
Enzyme Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
4.60
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