ELECTROPHYSIOLOGICAL ANALYSIS OF EXPERIMENTAL ULCERATION STAGES IN RATS.

Abstract

Research objective - to conduct electrophysiological analysis for experimental ulceration stages in rats studying changes in the electrical activity (EA) frequency characteristics (FCH) (frequency response) of the stomach and duodenum.

Materials and methods: The study was conducted in 5 experimental groups of outbred albino rats weighing 250-300 g with acetate stomach ulcerative injury (SUl). Using the method of direct multi-channel electrocardiography, we recorded the EA of gastric corpus (SB), its pyloric (PO) and the entry part of duodenum. We analyzed the frequency response of EA smooth muscles of stomach and duodenum during the period of seven days after the simulation SUI. The obtained by Shapiro-Wilk test data was distributed in accordance with statistically significant differences between samples - using the Student's t test. Correlation analysis was performed basing on the calculation of Pearson correlation coefficient.

Results: The average value of the frequency EA and TJ PO increased on the second day of the experiment 55.7% (p <0.01) and 53.0% (p <0.01), respectively, were down on the fourth day and then increased from 5 to 7 day of the experiment by 87.6% (p <0.01) in TJ and 89.8% (p <0.01) in the PO. The frequency EA LDK increased on the seventh day of the experiment 32,4% (p <0.05). The frequency EA LDK increased on the seventh day of the experiment 32.4% (p <0.05). Frequency EA inter-correlation among all the components of the gastroduodenal complex (GC) were the closest on the fourth day after inflicting the stomach ulcerative injury.

Conclusion: Frequency EA changes in correlation of components GC can serve as functional markers its integral condition in the process of experimental ulceration.