Alice Di Pierro , Heather Bondi , Chiara Monti , Luisa Pieroni , Enrico Cilio , Andrea Urbani , Tiziana Alberio , Mauro Fasano , Maurizio Ronci
{"title":"Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics","authors":"Alice Di Pierro , Heather Bondi , Chiara Monti , Luisa Pieroni , Enrico Cilio , Andrea Urbani , Tiziana Alberio , Mauro Fasano , Maurizio Ronci","doi":"10.1016/j.euprot.2016.02.002","DOIUrl":null,"url":null,"abstract":"<div><p>Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"11 ","pages":"Pages 1-3"},"PeriodicalIF":0.0000,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2016.02.002","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968516300150","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 2
Abstract
Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.
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