In Vitro AuNPs' Cytotoxicity and Their Effect on Wound Healing.

Q1 Engineering Nanobiomedicine Pub Date : 2015-01-01 DOI:10.5772/61132

Veronika Pivodová, Jana Franková, Adéla Galandáková, Jitka Ulrichová

{"title":"In Vitro AuNPs' Cytotoxicity and Their Effect on Wound Healing.","authors":"Veronika Pivodová, Jana Franková, Adéla Galandáková, Jitka Ulrichová","doi":"10.5772/61132","DOIUrl":null,"url":null,"abstract":"Recently, due to their unique properties, gold nanoparticles (AuNPs) have been used in many biological applications. However, little is known about their toxicity when they come into contact with a biological system. Based on the proposal that AuNPs can have a positive effect on wound healing, the present study investigated the influence of negatively-charged-surface AuNPs (average diameter 25-50 nm) on the viability of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK). Moreover, we evaluated the effect of AuNPs on the secretion of proteins involved in wound healing, such as interleukin-8 and - 12 (IL-8, IL-12), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), basic fibroblast grow factor (bFGF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). The results showed that AuNPs were not toxic to NHDF and NHEK. They showed a decrease in AuNPs' production of pro-inflammatory cytokines IL-6, IL-12 and TNF-α, as well as proteins involved in angiogenesis such as VEGF and bFGF. Thus, we suggest that AuNPs could have anti-inflammatory and anti-angiogenic activity.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"2 ","pages":"7"},"PeriodicalIF":0.0000,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/61132","citationCount":"52","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanobiomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5772/61132","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Engineering","Score":null,"Total":0}

引用次数: 52

Abstract

Recently, due to their unique properties, gold nanoparticles (AuNPs) have been used in many biological applications. However, little is known about their toxicity when they come into contact with a biological system. Based on the proposal that AuNPs can have a positive effect on wound healing, the present study investigated the influence of negatively-charged-surface AuNPs (average diameter 25-50 nm) on the viability of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK). Moreover, we evaluated the effect of AuNPs on the secretion of proteins involved in wound healing, such as interleukin-8 and - 12 (IL-8, IL-12), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), basic fibroblast grow factor (bFGF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). The results showed that AuNPs were not toxic to NHDF and NHEK. They showed a decrease in AuNPs' production of pro-inflammatory cytokines IL-6, IL-12 and TNF-α, as well as proteins involved in angiogenesis such as VEGF and bFGF. Thus, we suggest that AuNPs could have anti-inflammatory and anti-angiogenic activity.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

体外AuNPs的细胞毒性及其对伤口愈合的影响。

近年来，由于其独特的性质，金纳米颗粒(AuNPs)在许多生物领域得到了应用。然而，当它们与生物系统接触时，对它们的毒性知之甚少。基于AuNPs对伤口愈合的积极作用，本研究研究了带负电荷的表面AuNPs(平均直径25-50 nm)对正常人真皮成纤维细胞(NHDF)和正常人表皮角质形成细胞(NHEK)活力的影响。此外，我们还评估了AuNPs对参与伤口愈合的蛋白质分泌的影响，如白细胞介素-8和-12 (IL-8、IL-12)、肿瘤坏死因子-α (TNF-α)、血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)。结果表明，AuNPs对NHDF和NHEK无毒性。他们发现，AuNPs产生的促炎细胞因子IL-6、IL-12和TNF-α以及参与血管生成的蛋白质，如VEGF和bFGF，都有所减少。因此，我们认为AuNPs可能具有抗炎和抗血管生成活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Nanobiomedicine Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

14 weeks

期刊介绍： Nanobiomedicine is an international, peer-reviewed, open access scientific journal that publishes research in nanotechnology as it interfaces with fundamental studies in biology, as well as its application to the fields of medicine. Nanobiomedicine covers all key aspects of this research field, including, but not limited to, bioengineering, biophysics, physical and biological chemistry, and physiology, as well as nanotechnological applications in diagnostics, therapeutic application, preventive medicine, drug delivery, and monitoring of human disease. Additionally, theoretical and modeling studies covering the nanobiomedicine fields will be considered. All submitted articles considered suitable for Nanobiomedicine are subjected to rigorous peer review to ensure the highest levels of quality. The review process is carried out as quickly as possible to minimize any delays in the online publication of articles. Submissions are encouraged on all topics related to nanobiomedicine, and its clinical applications including but not limited to: Nanoscale-structured biomaterials, Nanoscale bio-devices, Nanoscale imaging, Nanoscale drug delivery, Nanobiotechnology, Nanorobotics, Nanotoxicology, Nanoparticles, Nanocarriers, Nanofluidics, Nanosensors (nanowires, nanophotonics), Nanosurgery (dermatology, gastroenterology, ophthalmology, etc), Nanocarriers commercialization of nanobiomedical technologies, Market trends in the nanobiomedicine space, Ethics and regulatory aspects of nanobiomedicine approval, New perspectives of nanobiomedicine in clinical diagnostics, BioMEMS, Nano-coatings, Plasmonics, Nanoscale visualization.