Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates.

Q3 Immunology and Microbiology Open Microbiology Journal Pub Date : 2018-05-31 eCollection Date: 2018-01-01 DOI:10.2174/1874285801812010181

Carolina Arango, Alejandro Acosta-Gonzalez, Claudia M Parra-Giraldo, Zilpa A Sánchez-Quitian, Russell Kerr, Luis E Díaz

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Abstract

Introduction: New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.

Material and methods: An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach.

Results: Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecium, extended-spectrum β-lactamase Klebsiella pnumoniae, and clinical isolates of Cladosporium cladosporioides and Epicoccum nigrum. Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS.

Conclusion: The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.

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对阿劳卡河（哥伦比亚）沉积物中放线菌群落的特征分析揭示了低丰度等位基因的抗菌潜力。

导言：为了快速有效地筛选出具有生物活性潜力的微生物，新策略应运而生。本研究表明，通过 MALDI-TOF MS 将物理化学预处理和微生物分类去重相结合，有助于检测具有抗菌剂来源潜力的低丰度放线菌：通过 MALDI-TOF MS 方法，采用理化预处理、生物筛选和分类去重等扩展培养策略，描述了哥伦比亚奥里诺基亚热带河流沉积物中一个未经研究的微生物群落：结果：生长了放线菌类分离物（790 株），并评估了它们对耐甲氧西林金黄色葡萄球菌、耐万古霉素粪肠球菌、广谱 β-内酰胺酶肺炎克雷伯菌以及 Cladosporium cladosporioides 和 Epicoccum nigrum 临床分离物的抗菌活性。根据 16S rDNA 分析，78 个属于链霉菌科的分离物具有抗菌活性，并通过 MALDI-TOF MS 被归类为低丰度放线菌：结果表明，通过 MALDI-TOF MS 对微生物藏品进行理化预处理和分类去重相结合，有助于检测具有抗菌剂来源潜力的低丰度放线菌。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.