Comparative iTRAQ proteomics revealed proteins associated with horn development in yak.

IF 2.1 3区生物学 Q3 BIOCHEMICAL RESEARCH METHODS Proteome Science Pub Date : 2018-07-24 eCollection Date: 2018-01-01 DOI:10.1186/s12953-018-0141-9

Mingna Li, Xiaoyun Wu, Xian Guo, Pengjia Bao, Xuezhi Ding, Min Chu, Chunnian Liang, Ping Yan

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引用次数: 13

Abstract

Background: The practice of dehorning yak raises animal safety concerns, which have been addressed by selective breeding to obtain genetically hornless yak. The POLLED locus in yak has been studied extensively; however, little is known regarding the proteins that regulate horn bud development.

Methods: A differential proteomic analysis was performed to compare the skin from the horn bud region of polled yak fetuses and the horn bud tissue of horned yak fetuses using isobaric tags for relative and absolute quantitation (iTRAQ) technology coupled with 2D LC-MS/MS.

Results: One hundred differentially abundant proteins (DAPs) were identified. Of these, 29 were up-regulated and 71 were down-regulated in skin from the horn bud region of polled fetuses when compared to the horn bud tissue of horned fetuses. Bioinformatics analyses showed that the up-regulated DAPs were mainly associated with metabolic activities, while the down-regulated DAPs were significantly enriched in cell adhesion and cell movement activities.

Conclusions: We concluded that some important proteins were associated with cell adhesion, cell motility, keratinocyte differentiation, cytoskeleton organization, osteoblast differentiation, and fatty acid metabolism during horn bud development. These results advance our understanding of the molecular mechanisms underlying horn development.

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比较iTRAQ蛋白质组学揭示了牦牛角发育的相关蛋白。

背景:牦牛去角的做法引起了动物安全问题，这已经通过选择性育种获得遗传上无角的牦牛来解决。牦牛的轮询位点已被广泛研究;然而，关于调节角芽发育的蛋白质知之甚少。方法:采用等压标签相对绝对定量(iTRAQ)技术，结合二维LC-MS/MS技术，采用差异蛋白质组学方法，比较了被调查牦牛胎角芽区皮肤和有角牦牛胎角芽组织。结果:鉴定出100个差异丰度蛋白(DAPs)。其中，与有角胎儿的角芽组织相比，投票胎儿的角芽区域皮肤中有29个上调，71个下调。生物信息学分析表明，上调的DAPs主要与代谢活性相关，而下调的DAPs在细胞粘附和细胞运动活性中显著富集。结论:在角芽发育过程中，一些重要的蛋白与细胞粘附、细胞运动、角质细胞分化、细胞骨架组织、成骨细胞分化和脂肪酸代谢有关。这些结果促进了我们对角发育的分子机制的理解。

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来源期刊

Proteome Science 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： Proteome Science is an open access journal publishing research in the area of systems studies. Proteome Science considers manuscripts based on all aspects of functional and structural proteomics, genomics, metabolomics, systems analysis and metabiome analysis. It encourages the submissions of studies that use large-scale or systems analysis of biomolecules in a cellular, organismal and/or environmental context. Studies that describe novel biological or clinical insights as well as methods-focused studies that describe novel methods for the large-scale study of any and all biomolecules in cells and tissues, such as mass spectrometry, protein and nucleic acid microarrays, genomics, next-generation sequencing and computational algorithms and methods are all within the scope of Proteome Science, as are electron topography, structural methods, proteogenomics, chemical proteomics, stem cell proteomics, organelle proteomics, plant and microbial proteomics. In spite of its name, Proteome Science considers all aspects of large-scale and systems studies because ultimately any mechanism that results in genomic and metabolomic changes will affect or be affected by the proteome. To reflect this intrinsic relationship of biological systems, Proteome Science will consider all such articles.