{"title":"RNAi Screening: Automated High-Throughput Liquid RNAi Screening in Caenorhabditis elegans","authors":"Sakthimala Jagadeesan, Abdul Hakkim","doi":"10.1002/cpmb.65","DOIUrl":null,"url":null,"abstract":"<p>RNAi is a powerful reverse genetics tool that has revolutionized genetic studies in model organisms. The bacteriovorous nematode <i>Caenorhabditis elegans</i> can be genetically manipulated by feeding it an <i>Escherichia coli</i> strain that expresses a double-stranded RNA (dsRNA) corresponding to a <i>C. elegans</i> gene, which leads to systemic silencing of the gene. This unit describes protocols for performing an automated high-throughput RNAi screen utilizing a full-genome <i>C. elegans</i> RNAi library. The protocols employ liquid-handling robotics and 96-well plates. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":10734,"journal":{"name":"Current Protocols in Molecular Biology","volume":"124 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmb.65","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmb.65","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 5
Abstract
RNAi is a powerful reverse genetics tool that has revolutionized genetic studies in model organisms. The bacteriovorous nematode Caenorhabditis elegans can be genetically manipulated by feeding it an Escherichia coli strain that expresses a double-stranded RNA (dsRNA) corresponding to a C. elegans gene, which leads to systemic silencing of the gene. This unit describes protocols for performing an automated high-throughput RNAi screen utilizing a full-genome C. elegans RNAi library. The protocols employ liquid-handling robotics and 96-well plates. © 2018 by John Wiley & Sons, Inc.
RNAi筛选:秀丽隐杆线虫的自动高通量液体RNAi筛选
RNAi是一种强大的反向遗传学工具，它已经彻底改变了模式生物的遗传研究。通过给线虫喂食一种表达与秀丽隐杆线虫基因相对应的双链RNA (dsRNA)的大肠杆菌菌株，可以对其进行遗传操纵，从而导致该基因的系统性沉默。本单元描述了使用全基因组秀丽隐杆线虫RNAi文库执行自动化高通量RNAi筛选的协议。该方案采用液体处理机器人和96孔板。©2018 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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