Distribution of tumor-infiltrating immune cells in glioblastoma.

Q1 Medicine CNS Oncology Pub Date : 2018-12-01 Epub Date: 2018-10-09 DOI:10.2217/cns-2017-0037

Enrique Orrego, Carlos A Castaneda, Miluska Castillo, Luis A Bernabe, Sandro Casavilca, Arnab Chakravarti, Wei Meng, Pamela Garcia-Corrochano, Maria R Villa-Robles, Rocio Zevallos, Omar Mejia, Pedro Deza, Carolina Belmar-Lopez, Luis Ojeda

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引用次数: 40

Abstract

Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma.

Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis.

Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4⁺ was associated with unmethylated MGMT (p = 0.016). Higher CD8⁺ was associated with larger tumoral size (p = 0.027). Higher CD163⁺ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4⁺ (p < 0.05) were associated with longer overall survival.

Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.

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肿瘤浸润免疫细胞在胶质母细胞瘤中的分布。

目的:探讨胶质母细胞瘤浸润性免疫细胞水平的相关特征。方法:通过H&E染色检测肿瘤浸润淋巴细胞(TILs)，通过数字化分析免疫组化检测肿瘤浸润淋巴细胞(TILs) (CD3、CD4、CD8、CD20)和巨噬细胞(CD68、CD163)水平。结果:CD68(9.1%)、CD163(2.2%)、CD3(1.6%)和CD8(1.6%)密度最高。CD4+升高与MGMT未甲基化相关(p = 0.016)。CD8+水平越高，肿瘤大小越大(p = 0.027)。CD163+水平越高，年龄越大(p = 0.044)，递归划分分析= 4。结论:巨噬细胞多于TILs。一些子集与临床特征有关。

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