Rapid and Efficient Genetic Transformation of Sorghum via Agrobacterium-Mediated Method

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2018-10-12 DOI:10.1002/cppb.20077

Phat Tien Do, Hyeyoung Lee, Kimberly Nelson-Vasilchik, Albert Kausch, Zhanyuan J. Zhang

引用次数: 11

Abstract

Genetic transformation via Agrobacterium-mediated methodology has been used in many sorghum studies. However, the transformation efficiency still varies significantly due to high dependence on sorghum genotypes and technical expertise. In this article, we describe a sorghum transformation procedure in sufficient detail using a public genotype, P898012. This system utilizes a standard binary transgenic vector carrying the bar gene as a selectable marker and immature embryos as starting explants. Glufosinate is employed as the selective agent during callus and shoot induction. This procedure is relatively rapid, efficient, highly reproducible, and should be applicable for many other sorghum genotypes. © 2018 by John Wiley & Sons, Inc.

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农杆菌介导的高粱快速高效遗传转化研究

通过农杆菌介导的遗传转化方法已在许多高粱研究中使用。然而，由于对高粱基因型和技术专长的高度依赖，转化效率仍然存在显著差异。在这篇文章中，我们用一个公开基因型P898012详细描述了高粱的转化过程。该系统采用标准的二元转基因载体携带bar基因作为选择标记，未成熟胚胎作为起始外植体。草甘膦作为愈伤组织和芽诱导的选择性剂。该方法相对快速、高效、可重复性高，适用于许多其他高粱基因型。©2018 by John Wiley &儿子,Inc。

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Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.