A Torgomyan, S Adamyan, H Ghambaryan, H Hasratyan, D Khudaverdyan, C Archer
{"title":"Parathyroid hormone influence on chondrogenic progenitor cells differentiation.","authors":"A Torgomyan, S Adamyan, H Ghambaryan, H Hasratyan, D Khudaverdyan, C Archer","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The anabolic effects require brief exposures to higher than average PTH concentrations. The catabolic effects result from pathological conditions in which parathyroid glands secrete too much hormone continuously at a sustained level. The aim of the present study was observance of intermittent (I-PTH) and continuous (C-PTH) PTH 1-34 administration influence on bovine chondrogenic progenitor cells (CPC) differentiation. Alcian blue was used to determine proteoglycan accumulation in CPC monolayer cultures. Thus in plates containing CPCs in C-PTH media chondrogenic differentiation was indicated by intensive proteoglycan accumulation in extracellular matrix. CPCs monolayer cultured in osteogenic media were subjected to Alizarin Red staining for matrix mineralization detection. Intense staining was revealed in I-PTH cells in comparison with C-PTH plates. The data obtained by monolayer cultures histological staining were confirmed by PCR analysis.</p>","PeriodicalId":23230,"journal":{"name":"TSitologiia i genetika","volume":"50 5","pages":"43-7"},"PeriodicalIF":0.0000,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"TSitologiia i genetika","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The anabolic effects require brief exposures to higher than average PTH concentrations. The catabolic effects result from pathological conditions in which parathyroid glands secrete too much hormone continuously at a sustained level. The aim of the present study was observance of intermittent (I-PTH) and continuous (C-PTH) PTH 1-34 administration influence on bovine chondrogenic progenitor cells (CPC) differentiation. Alcian blue was used to determine proteoglycan accumulation in CPC monolayer cultures. Thus in plates containing CPCs in C-PTH media chondrogenic differentiation was indicated by intensive proteoglycan accumulation in extracellular matrix. CPCs monolayer cultured in osteogenic media were subjected to Alizarin Red staining for matrix mineralization detection. Intense staining was revealed in I-PTH cells in comparison with C-PTH plates. The data obtained by monolayer cultures histological staining were confirmed by PCR analysis.
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