Morphogenesis in vitro in inbreds of maize Lancaster heterotic group.

Abstract

The genotypic features of morphogenesis and regeneration in vitro for five maize inbreds of perspective breeding Lancaster heterotic group compared to representatives of others heterotic groups – PLS61, A188 and Chi31 were studied. It was identified that the ratio of such types of morphogenesis as organogenesis and embryoidogenesis in callus culture was determined by the explant genotype and the concentration of sucrose in the medium for callusogenesis. The frequency of embryoidogenesis as the most effective type of morphogenesis for further regeneration in Lancaster inbreds averaged about 40.0 ± 12.8 %, while for other heterotic groups it was only 14.0 ± 4.0 %. For Lancaster heterotic group sucrose at the concentration of 30 g/l in the medium for callusogenesis provided further regeneration through embryoidogenesis at the level of 26.5 ± 15.4 %, but sucrose at the concentration of 60 g/l provided it at 57.7 ± 19.8 %. For inbreds which represent other heterotic groups sucrose content in the medium for callusogenesis did not affect further regenaration, the level of embryoidogenesis at 30 and 60 g/l sucrose amounted 11.0 ± 7.0 and 15.0 ± 4.8 % correspondingly.