[Effects of β-asarone on Epithelial-Mesenchymal Transition of Gastric Cancer in Nude Mice].

Abstract

Objective To observe the effects of β-asarone on epithelial-mesenchymal transition (EMT) of human gastric cancer MGC-803 cells in BALB/c nude mice,and to study its possible molecular mechanism. Methods Gastric cancer MGC-803 cells were subcutaneously inoculated to nude mice for preparing transplanted tumor model. Totally 24 nude mice were then divided into the negative control group (model) , the positive control group (5-FU,25 mg/kg) , the high dose β-asarone group (100 mg/ kg) , the low dose β-asarone group (50 mg/kg) , 8 in each group. Corresponding medicines were adminis- tered to rats in respective group by gastrogavage, once per day for 10 successive days. The mice were sacrificed at the end of the intervention, and the tumor inhibition rate was calculated. The expressions of E-cadherin, N-cadherin, Snail, phosphatidylinositol 3-kinase (PI3K), phosphorylation of phosphatidylinositol 3-kinase ( p-PI3K ) , serine/threonine kinase ( AKT) , phosphorylation of serine/threonine kinase (p-AKT) were detected by Real-time PCR and Western Blot. Results Compared with the model group, the volume of transplanted tumor was obviously reduced in 5-FU group and β-asarone groups from day7 to day 11 (P <0.05). Protein and mRNA expressions of N-cadherin, Snail, p-PI3K, p-AKT decreased, and protein and mRNA expressions of E-cadherin increased in 5-FU group and β-asarone groups (P < 0. 05). Conclusions β-asarone could inhibit proliferation ability of gastric cancer cells, and its mecha- nism might be associated with down-regulating P13K/AKT signal pathway of gastric cancer cells and re- straining EMT of gastric cancer cells.