Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues.

IF 1 4区医学 Q4 BIOPHYSICS Biorheology Pub Date : 2019-01-01 DOI:10.3233/BIR-180185

Asma Alanazi, Hafsa Munir, Mohammed Alassiri, Lewis S C Ward, Helen M McGettrick, Gerard B Nash

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引用次数: 11

Abstract

Background: Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood.

Objective: We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue.

Methods: MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time. MSC migration through 8 μm pore filters coated with the same proteins was analysed.

Results: The number of MSC adhering to collagen was greater than fibronectin, decreased as wall shear rate increased from 17 to 70 s-1, and was in the order UCMSC>BMMSC>TBMSC. Conversely, spreading was more effective on fibronectin and was in the order BMMSC>TBMSC≥UCMSC. Migration was promoted by coating the lower surface of filters with either matrix protein, with UCMSC migrating more efficiently than BMMSC.

Conclusions: MSC show origin-dependent variations in their efficiency of capture from flow and subsequent spreading or ability to migrate on matrix proteins. UCMSC showed most efficient capture from flow, which was followed by less spreading, but more rapid migration. These responses might be associated with more effective delivery from the circulation into damaged tissue.

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不同组织间充质干细胞粘附和迁移特性的比较。

背景:间充质干细胞(MSC)用于治疗，通常通过注射到血液中。目的:比较脐带间充质干细胞(UCMSC)、骨髓间充质干细胞(BMMSC)和骨小梁间充质干细胞(TBMSC)的粘附和迁移特性，探讨其对损伤组织递送的影响。方法:骨髓间充质干细胞通过涂有基质蛋白、胶原或纤维连接蛋白或白蛋白的玻璃毛细血管灌注。显微镜下对贴壁细胞进行计数，并分析其随时间的扩散情况。分析了MSC通过包被相同蛋白的8 μm孔过滤器的迁移。结果:粘附于胶原的间充质干细胞数量大于纤维连接蛋白数量，随壁剪切速率的增加而减少，大小为UCMSC>BMMSC>TBMSC。相反，扩散对纤维连接蛋白更有效，其顺序为BMMSC>TBMSC≥UCMSC。两种基质蛋白均可促进滤膜下表面的迁移，且UCMSC的迁移效率高于BMMSC。结论:MSC在其从流动中捕获的效率和随后的扩散或在基质蛋白上迁移的能力方面表现出来源依赖性的变化。UCMSC最有效地捕获了流动，其次是传播较少，但迁移速度较快。这些反应可能与血液循环更有效地将药物输送到受损组织有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.