Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI:10.1016/j.jgeb.2018.02.012
Rania Samy Hanafy, Samia Ageeb Akladious
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引用次数: 56

Abstract

This experiment assessed the biochemical changes in fenugreek plants exposed to gamma radiation. Two pot experiments were carried out during two growing seasons of 2015 and 2016. Seeds were subjected to five doses of gamma irradiation (25, 50, 100, 200 and 400 Gy) and were immediately planted into soil pots in a greenhouse. The experimental analysis was performed in M1 and M2 generations. Significant differences between irradiated and control plants were detected for most studied characters in M1 and M2 generations. It was demonstrated that low doses of gamma irradiation led to gradually increases in growth, yield characters, leaf soluble protein concomitantly with increases in the contents of phenolic and flavonoids compounds particularly at 100 Gy. These changes were accompanied by a substantial increase in ascorbic acid, α-tocopherol and retinol contents. Proline content was increased under all doses of gamma rays in M1 generation and the highest amount of proline was obtained at 200 Gy with visible decrease in M2 generation under the same dose. Meanwhile, the highest dose of gamma radiation (400 Gy) decreased all the studied parameters in both mutagenic generations as compared with control plants. In addition, gamma irradiation doses induced changes in DNA profile on using five primers and caused the appearance and disappearance of DNA polymorphic bands with variation in their intensity. These findings confirm the effectiveness of relatively low doses of gamma rays on improving the physiological and biochemical criteria of fenugreek plants.

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伽马辐射对葫芦巴(Trigonella foenum-graecum L.)植物影响的生理和分子研究
本实验评估了伽马辐射下葫芦巴植物的生化变化。在2015年和2016年两个生长季节进行了两次盆栽试验。种子受到五次剂量的伽马辐照(25、50、100、200和400 Gy),并立即种植在温室的土壤花盆中。对M1代和M2代进行了实验分析。辐照植株和对照植株在M1和M2代的大部分性状上存在显著差异。结果表明,在低剂量辐照下,特别是在100 Gy辐照下,幼苗的生长、产量、叶可溶性蛋白逐渐增加,酚类化合物和黄酮类化合物的含量也随之增加。这些变化伴随着抗坏血酸、α-生育酚和视黄醇含量的显著增加。在所有剂量下,M1代的脯氨酸含量都有所增加,在200 Gy时脯氨酸含量最高,而M2代在相同剂量下脯氨酸含量明显下降。同时,与对照植株相比,最高剂量(400 Gy)降低了诱变世代的所有研究参数。此外,γ辐照剂量引起了5种引物DNA谱的变化,并引起DNA多态性带的出现和消失,随其强度的变化。这些发现证实了相对低剂量伽马射线对改善葫芦巴植物生理生化指标的有效性。
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来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
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