PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2019-01-01 DOI:10.1247/csf.18030
Yuanyu Wang, Weili Sun, Jiankai Yang, Liang Yang, Chen Li, Hongjiang Liu, Xiaopeng Liu, Baohua Jiao
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引用次数: 15

Abstract

PUM2, an RNA binding protein, is known to promote stem cell proliferation via repressing expressions of cell cycle genes. Similar with stem cells, malignant cells are characterized by unlimited proliferation and remote migration. However, roles of PUM2 in cancer development are controversial. Here, we investigated PUM2's role in glioblastoma development and its relationship with the cell cycle regulator BTG1. Immunoblotting and RT-qPCR were used to evaluate protein expression level and transcript level, respectively. ShRNAs were designed to knock down PUM2 and BTG1 expression. CCK-8 assay was used to evaluate cell viability. Cell migration assay and evasion assay were used to evaluate metastatic capability of glioblastoma cell. RNA pull-down assay and RNA immunoprecipitation assay were used to test the interaction between PUM2 and BTG1 3'UTR. PUM2 expression is elevated in glioblastoma tumor tissues as well as glioblastoma cell lines. PUM2 knockdown remarkably suppresses glioblastoma cell proliferation and migration. In addition, PUM2 knockdown increases BTG1 expression. RNA pull-down assay and RNA immunoprecipitation assay show PUM2 binds to BTG1 3'UTR directly. Furthermore, knockdown of BTG1 reverses the effect of PUM2 knockdown on glioblastoma cell proliferation and migration. Our results suggest that PUM2 promote glioblastoma development via repressing BTG1 expression.Key words: PUM2, BTG1, glioblastoma, cell proliferation, metastasis.

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PUM2通过抑制BTG1表达促进胶质母细胞瘤细胞增殖和迁移。
PUM2是一种RNA结合蛋白,已知通过抑制细胞周期基因的表达来促进干细胞增殖。与干细胞相似,恶性细胞具有无限增殖和远程迁移的特点。然而,PUM2在癌症发展中的作用仍存在争议。在这里,我们研究了PUM2在胶质母细胞瘤发育中的作用及其与细胞周期调节因子BTG1的关系。采用免疫印迹法和RT-qPCR法分别检测蛋白表达水平和转录物水平。shrna被设计用于敲低PUM2和BTG1的表达。CCK-8法测定细胞活力。采用细胞迁移法和逃避法评价胶质母细胞瘤细胞的转移能力。采用RNA拉下法和RNA免疫沉淀法检测PUM2与BTG1 3'UTR的相互作用。PUM2在胶质母细胞瘤肿瘤组织和胶质母细胞瘤细胞系中表达升高。PUM2敲低显著抑制胶质母细胞瘤细胞的增殖和迁移。此外,PUM2敲低会增加BTG1的表达。RNA拉下实验和RNA免疫沉淀实验显示PUM2直接结合BTG1 3'UTR。此外,BTG1的敲低逆转了PUM2敲低对胶质母细胞瘤细胞增殖和迁移的影响。我们的研究结果表明PUM2通过抑制BTG1的表达促进胶质母细胞瘤的发展。关键词:PUM2, BTG1,胶质母细胞瘤,细胞增殖,转移
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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