Sodium Oleate Increases Ampicillin Sensitivity in Methylophilus quaylei Biofilms on Teflon and Polypropylene.

IF 2.2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Current pharmaceutical biotechnology Pub Date : 2019-01-01 DOI:10.2174/1389201020666190222191656

Abir M H A Mohamed, Shevlyagina N Vladimirovna, Zhukhovitsky V Grigorievich, Pshenichnikova A Borisovna, Shvets V Ivanovich

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引用次数: 1

Abstract

Background: Drug combination is a new therapy to improve antibiotic deficiency treatment towards biofilm resistance.

Objective: This study was conducted to determine the potential effect of sodium oleate to inhibit established biofilms of two strains, methylotrophic bacteria Methylophilus quaylei in combination with ampicillin. Minimum inhibitory concentration (MIC) of ampicillin was determined and added in combination with sodium oleate and examined on planktonic and established biofilms of two strains M. quaylei were characterized by different properties of cell surface hydrophobicity.

Methods: The effect on biofilms was evaluated by the number of colony forming units (CFUs), crystal violet assay, light and scanning electron microscopy.

Results: The study demonstrates that sodium oleate has a promoting activity against planktonic growth of M. quaylei strains and has a slight inhibitory effect on biofilm. Addition of sodium oleate enhances the bactericidal effect of ampicillin against biofilm cells. Combination of ampicillin 0.1 mg/ml (MIC) and sodium oleate 0.03 mg/ml showed a remarkable destruction effect on established biofilms.

Discussion: Combination of ampicillin 0.1 mg/ml (MIC) and sodium oleate 0.03 mg/ml showed a remarkable destruction effect on established biofilms. Overall, results indicated that sodium oleate in combination with ampicillin enhances the inhibition of M. quaylei biofilms and this combination can be utilized for combating bacterial biofilm resistance.

Conclusion: Overall, results indicated that sodium oleate in combination with ampicillin enhances the inhibition of M. quaylei biofilms and this combination can be utilized for combating bacterial biofilm resistance.

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油酸钠增加嗜甲基蝇在聚四氟乙烯和聚丙烯上的氨苄西林敏感性。

背景:联合用药是改善抗生素缺乏治疗生物膜耐药的新方法。目的:研究油酸钠与氨苄西林联用对两种甲基营养细菌——嗜甲氧杆菌形成的生物膜的潜在抑制作用。测定了氨苄西林的最低抑菌浓度(MIC)，并与油酸钠联合添加，在浮游生物膜和已建立的生物膜上对两种菌株的细胞表面疏水性进行了研究。方法:采用菌落形成单位数(cfu)、结晶紫法、光镜、扫描电镜等方法评价其对生物膜的影响。结果:油酸钠对quaylei M.菌株的浮游生长有促进作用，对生物膜有轻微抑制作用。油酸钠的加入增强了氨苄西林对生物膜细胞的杀菌作用。氨苄西林0.1 mg/ml (MIC)和油酸钠0.03 mg/ml联合使用对已建立的生物膜有显著的破坏作用。讨论:氨苄西林0.1 mg/ml (MIC)与油酸钠0.03 mg/ml联合使用对已建立的生物膜有显著的破坏作用。综上所述，油酸钠与氨苄西林联用可增强对M. quaylei生物膜的抑制作用，该联用可用于对抗细菌生物膜耐药性。结论:油酸钠与氨苄西林联用可增强对细菌生物膜的抑制作用，可用于抗细菌生物膜耐药。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current pharmaceutical biotechnology 医学-生化与分子生物学

CiteScore

5.60

自引率

3.60%

发文量

203

审稿时长

6 months

期刊介绍： Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal includes timely in-depth reviews, original research articles and letters written by leaders in the field, covering a range of current topics in scientific areas of Pharmaceutical Biotechnology. Invited and unsolicited review articles are welcome. The journal encourages contributions describing research at the interface of drug discovery and pharmacological applications, involving in vitro investigations and pre-clinical or clinical studies. Scientific areas within the scope of the journal include pharmaceutical chemistry, biochemistry and genetics, molecular and cellular biology, and polymer and materials sciences as they relate to pharmaceutical science and biotechnology. In addition, the journal also considers comprehensive studies and research advances pertaining food chemistry with pharmaceutical implication. Areas of interest include: DNA/protein engineering and processing Synthetic biotechnology Omics (genomics, proteomics, metabolomics and systems biology) Therapeutic biotechnology (gene therapy, peptide inhibitors, enzymes) Drug delivery and targeting Nanobiotechnology Molecular pharmaceutics and molecular pharmacology Analytical biotechnology (biosensing, advanced technology for detection of bioanalytes) Pharmacokinetics and pharmacodynamics Applied Microbiology Bioinformatics (computational biopharmaceutics and modeling) Environmental biotechnology Regenerative medicine (stem cells, tissue engineering and biomaterials) Translational immunology (cell therapies, antibody engineering, xenotransplantation) Industrial bioprocesses for drug production and development Biosafety Biotech ethics Special Issues devoted to crucial topics, providing the latest comprehensive information on cutting-edge areas of research and technological advances, are welcome. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.