HPV16 variant analysis in primary and recurrent CIN2/3 lesions demonstrates presence of the same consensus variant

Pascal van der Weele , Audrey J. King , Chris J.L.M. Meijer , Renske D.M. Steenbergen
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引用次数: 4

Abstract

Introduction

Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5–15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions.

Methods

Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized.

Results

From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline.

Conclusion

Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.

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原发性和复发性CIN2/3病变中的HPV16变异分析表明存在相同的共识变异
宫颈上皮内病变复发(rCIN2/3)在CIN2/3治疗后发生在5-15%的病例。rCIN2/3可以由CIN2/3的不完全切除引起,其中相同的HPV类型和变体仍然存在。rCIN2/3也可能发生在与初始病变相同HPV类型的不同HPV变体的新感染后。本研究调查了基线CIN2/3和rCIN2/3病变配对HPV16阳性刮痕中的HPV16共识变异。方法选择治疗6个月或12个月后CIN2/3 和CIN2/3的女性HPV16阳性宫颈刮伤,进行全基因组扩增和Illumina测序。序列比较和核苷酸随时间变化的特征。结果在14个配对样本中,10个在基线CIN2/3和rCIN2/3有相同的共识变异。与基线相比,四个配对样本在复发性疾病中显示一到三个核苷酸变异。结论HPV16基线CIN2/3和rCIN2/3配对病变治疗后,在刮擦中发现相同或几乎相同的HPV16一致变异体,提示在两种病变中发现相同HPV型别时无需进行HPV变异体分析。这些结果表明要么不完全切除基线CIN2/3,要么无法清除原始HPV感染。
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期刊介绍: The official Journal of the International Papillomavirus Society Papillomavirus Research (PVR), the Journal of HPV and other Small DNA Tumor Viruses publishes innovative papers related to all aspects of papillomaviruses and other small DNA tumor viruses. The official journal of the International Papillomavirus Society, PVR is an open access publication that aims to bring together virologists, immunologists, epidemiologists and clinicians working in the booming field of HPV and animal papillomaviruses, polyomaviruses and other small DNA tumor viruses and their associated diseases, in order to foster and facilitate interdisciplinary communication. The journal welcomes original research articles, reviews, short communications, opinion articles and regional update reports on papillomaviruses and other tumor viruses in the following sections: a. Biology of papillomaviruses and related viruses from life cycle to cancer b. Epidemiology etiology and natural history studies c. Natural and induced immunity including vaccine research d. Intervention studies and strategies including i. Clinical studies and trials ii. HPV treatments iii. HPV vaccination programs iv. Diagnostics and screening e. Infection and disease prevention, modeling studies f. Guidelines and public health recommendations g. HPV Studies in special populations Regional and local studies on these viruses.
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