Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice.

IF 4.7 2区 心理学 Q1 BEHAVIORAL SCIENCES Behavioral and Brain Functions Pub Date : 2019-05-07 DOI:10.1186/s12993-019-0160-4
Hareram Birla, Chetan Keswani, Sachchida Nand Rai, Saumitra Sen Singh, Walia Zahra, Hagera Dilnashin, Aaina Singh Rathore, Surya Pratap Singh
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Abstract

Background: Bisphenol A (BPA), a major endocrine disruptor and a xenobiotic compound is used abundantly in the production of polycarbonate plastics and epoxy resins. Human exposure to this compound is primarily via its leaching from the protective internal epoxy resin coatings of containers into the food and beverages. In addition, the plastics used in dental prostheses and sealants also contain considerable amount of BPA and have a high risk of human exposure. Since it is a well-known endocrine disruptor and closely mimics the molecular structure of human estrogen thereby impairing learning and memory. Withania somnifera (Ws), commonly known as Ashwagandha is known for its varied therapeutic uses in Ayurvedic system of medicine. The present study was undertaken to demonstrate the impairment induced by BPA on the spatial learning, working memory and its alleviation by Ws in Swiss albino mice. The study was conducted on thirty Swiss albino mice, randomly distributed among three groups: control, BPA and BPA + Ws. The behavioral recovery after treatment with Ws was investigated using the Y-maize and Morris water maize test. Whereas, for the estimation of recovery of NMDA receptor which is related to learning and memory in hippocampus region by western blot and immunohistochemistry. Furthermore, the oxidative stress and antioxidant level was assessed by biochemical tests like MDA, SOD and catalase.

Results: The study revealed that administration of Ws alleviated the behavioral deficits induced by BPA. Alongside, Ws treatment reinstated the number of NMDA receptors in hippocampus region and showed anti-oxidative property while ameliorating the endogenous anti-oxidant level in the brain.

Conclusion: These findings suggest that Ws significantly ameliorates the level of BPA intoxicated oxidative stress thereby potentially treating cognitive dysfunction which acts as the primary symptom in a number of neurodegenerative diseases.

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睡茄对双酚 A 诱导的小鼠认知功能障碍和氧化应激的神经保护作用
背景:双酚 A(BPA)是一种主要的内分泌干扰物和异生物化合物,被大量用于聚碳酸酯塑料和环氧树脂的生产。人类接触这种化合物的主要途径是从容器内部环氧树脂保护涂层中渗入食品和饮料中。此外,义齿和密封剂中使用的塑料也含有大量双酚 A,人体接触的风险很高。由于双酚 A 是一种众所周知的内分泌干扰物,与人体雌激素的分子结构相似,因此会影响学习和记忆。Withania somnifera(Ws),俗称 Ashwagandha,因其在阿育吠陀医学体系中的多种治疗用途而闻名。本研究旨在证明双酚 A 对瑞士白化小鼠空间学习和工作记忆的影响,以及 Ws 对这种影响的缓解作用。研究对象是 30 只瑞士白化小鼠,随机分为三组:对照组、双酚 A 组和双酚 A + Ws 组。使用 Ws 治疗后,小鼠的行为恢复情况通过 Y-玉米试验和莫里斯水玉米试验进行了调查。此外,还通过 Western 印迹和免疫组化技术评估了海马区与学习和记忆有关的 NMDA 受体的恢复情况。此外,还通过 MDA、SOD 和过氧化氢酶等生化测试评估了氧化应激和抗氧化水平:研究结果表明,服用 Ws 可减轻双酚 A 引起的行为缺陷。此外,Ws 还能恢复海马区 NMDA 受体的数量,并显示出抗氧化特性,同时改善大脑中的内源性抗氧化剂水平:这些研究结果表明,Ws 能明显改善双酚 A 中毒的氧化应激水平,从而有可能治疗作为多种神经退行性疾病主要症状的认知功能障碍。
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来源期刊
Behavioral and Brain Functions
Behavioral and Brain Functions 医学-行为科学
CiteScore
5.90
自引率
0.00%
发文量
11
审稿时长
6-12 weeks
期刊介绍: A well-established journal in the field of behavioral and cognitive neuroscience, Behavioral and Brain Functions welcomes manuscripts which provide insight into the neurobiological mechanisms underlying behavior and brain function, or dysfunction. The journal gives priority to manuscripts that combine both neurobiology and behavior in a non-clinical manner.
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