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{"title":"Recombinant Viral Vectors as Neuroscience Tools","authors":"Shih-Heng Chen, Juhee Haam, Mitzie Walker, Erica Scappini, John Naughton, Negin P. Martin","doi":"10.1002/cpns.67","DOIUrl":null,"url":null,"abstract":"<p>Recombinant viruses are highly efficient vehicles for <i>in vivo</i> gene delivery. Viral vectors expand the neurobiology toolbox to include direct and rapid anterograde, retrograde, and trans-synaptic delivery of tracers, sensors, and actuators to the mammalian brain. Each viral type offers unique advantages and limitations. To establish strategies for selecting a suitable viral type, this article aims to provide readers with an overview of viral recombinant technology, viral structure, tropism, and differences between serotypes and pseudotypes for three of the most commonly used vectors in neurobiology research: adeno-associated viruses, retro/lentiviruses, and glycoprotein-deleted rabies viruses. © 2019 by John Wiley & Sons, Inc.</p>","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.67","citationCount":"23","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Neuroscience","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpns.67","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Neuroscience","Score":null,"Total":0}
引用次数: 23
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Abstract
Recombinant viruses are highly efficient vehicles for in vivo gene delivery. Viral vectors expand the neurobiology toolbox to include direct and rapid anterograde, retrograde, and trans-synaptic delivery of tracers, sensors, and actuators to the mammalian brain. Each viral type offers unique advantages and limitations. To establish strategies for selecting a suitable viral type, this article aims to provide readers with an overview of viral recombinant technology, viral structure, tropism, and differences between serotypes and pseudotypes for three of the most commonly used vectors in neurobiology research: adeno-associated viruses, retro/lentiviruses, and glycoprotein-deleted rabies viruses. © 2019 by John Wiley & Sons, Inc.
重组病毒载体作为神经科学工具
重组病毒是体内基因传递的高效载体。病毒载体扩展了神经生物学工具箱，包括直接和快速的顺行、逆行和跨突触传递示踪剂、传感器和致动器到哺乳动物的大脑。每种病毒类型都有其独特的优点和局限性。为了确定选择合适病毒类型的策略，本文旨在为读者提供三种最常用的神经生物学研究载体:腺相关病毒、复古/慢病毒和糖蛋白缺失狂犬病毒的病毒重组技术、病毒结构、趋向性以及血清型和伪型之间的差异的概述。©2019 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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