Bivalency in Drosophila embryos is associated with strong inducibility of Polycomb target genes.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Fly Pub Date : 2019-03-01 Epub Date: 2019-05-25 DOI:10.1080/19336934.2019.1619438

Arslan Akmammedov, Marco Geigges, Renato Paro

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引用次数: 7

Abstract

Polycomb group (PcG) and Trithorax group (TrxG) proteins orchestrate development of a multicellular organism by faithfully maintaining cell fate decisions made early in embryogenesis. An important chromatin mark connected to PcG/TrxG regulation is bivalent domains, the simultaneous presence of H3K27me3 and H3K4me3 on a given locus, originally identified in mammalian embryonic stem cells but considered to be absent in invertebrates. Here, we provide evidence for the existence of bivalency in fly embryos. Using a recently described PcG reporter fly line, we observed a strong reporter inducibility in the embryo and its sharp decrease in larval and adult stages. Analysis of the chromatin landscape of the reporter revealed a strong signal for the repressive PcG mark, H3K27me3, in all three developmental stages and, surprisingly, a strong signal for a transcriptionally activating H3K4me3 mark in the embryo. Using re-chromatin immunoprecipitation experiments, bivalent domains were also uncovered at endogenous PcG targets like the Hox genes.

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果蝇胚胎的二价性与Polycomb靶基因的强诱导性有关。

Polycomb group (PcG)和Trithorax group (TrxG)蛋白通过忠实地维持胚胎发生早期的细胞命运决定来协调多细胞生物的发育。与PcG/TrxG调控相关的一个重要染色质标记是二价结构域，即H3K27me3和H3K4me3同时存在于给定位点上，最初在哺乳动物胚胎干细胞中发现，但被认为在无脊椎动物中不存在。本研究为蝇胚中存在二价性提供了证据。利用最近描述的PcG报告蝇系，我们观察到报告蝇在胚胎中具有很强的诱导性，并且在幼虫期和成虫期急剧下降。对报告基因染色质结构的分析显示，抑制PcG标记H3K27me3在所有三个发育阶段都有很强的信号，令人惊讶的是，胚胎中转录激活H3K4me3标记也有很强的信号。利用重染色质免疫沉淀实验，在内源性PcG靶点(如Hox基因)上也发现了二价结构域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fly 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.