{"title":"Nicole Le Douarin and the use of quail-chick chimeras to study the developmental fate of neural crest and hematopoietic cells","authors":"Domenico Ribatti","doi":"10.1016/j.mod.2019.103557","DOIUrl":null,"url":null,"abstract":"<div><p>The quail-chick chimera marking system, devised in 1969, gave a new impetus to the analysis of cell migrations and interactions in the developing nervous, immune and hematopoietic systems. The method is based on the observation that the constitutive heterochromatin in all embryonic and adult cells of the quail is condensed in one large mass in the centre of the nucleus and is associated with the nucleolus, making this organelle strongly stained with the Feulgen–Rossenbeck reaction. The association of cells or rudiments from two avian species, advocated as a means to identify cells that migrate during embryogenesis, was rapidly recognized in this context as a useful tool for the study of many developmental biology problems. This article summarizes the fundamental contribution of Nicole Le Douarin to the discovery and the application of this technique over the last 40 years.</p></div>","PeriodicalId":49844,"journal":{"name":"Mechanisms of Development","volume":"158 ","pages":"Article 103557"},"PeriodicalIF":2.6000,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mod.2019.103557","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mechanisms of Development","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925477319300942","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 5
Abstract
The quail-chick chimera marking system, devised in 1969, gave a new impetus to the analysis of cell migrations and interactions in the developing nervous, immune and hematopoietic systems. The method is based on the observation that the constitutive heterochromatin in all embryonic and adult cells of the quail is condensed in one large mass in the centre of the nucleus and is associated with the nucleolus, making this organelle strongly stained with the Feulgen–Rossenbeck reaction. The association of cells or rudiments from two avian species, advocated as a means to identify cells that migrate during embryogenesis, was rapidly recognized in this context as a useful tool for the study of many developmental biology problems. This article summarizes the fundamental contribution of Nicole Le Douarin to the discovery and the application of this technique over the last 40 years.
1969年设计的鹌鹑-小鸡嵌合体标记系统为神经系统、免疫系统和造血系统的细胞迁移和相互作用的分析提供了新的推动力。该方法是基于观察到鹌鹑所有胚胎和成年细胞中的组成异染色质在细胞核中心凝聚成一个大团块，并与核仁相关，使该细胞器被Feulgen-Rossenbeck反应强烈染色。来自两种鸟类的细胞或雏形的关联，被认为是鉴定胚胎发生过程中迁移细胞的一种手段，在这种背景下，迅速被认为是研究许多发育生物学问题的有用工具。本文总结了Nicole Le Douarin在过去40 年中对该技术的发现和应用的基本贡献。
期刊介绍：
Mechanisms of Development is an international journal covering the areas of cell biology and developmental biology. In addition to publishing work at the interphase of these two disciplines, we also publish work that is purely cell biology as well as classical developmental biology.
Mechanisms of Development will consider papers in any area of cell biology or developmental biology, in any model system like animals and plants, using a variety of approaches, such as cellular, biomechanical, molecular, quantitative, computational and theoretical biology.
Areas of particular interest include:
Cell and tissue morphogenesis
Cell adhesion and migration
Cell shape and polarity
Biomechanics
Theoretical modelling of cell and developmental biology
Quantitative biology
Stem cell biology
Cell differentiation
Cell proliferation and cell death
Evo-Devo
Membrane traffic
Metabolic regulation
Organ and organoid development
Regeneration
Mechanisms of Development does not publish descriptive studies of gene expression patterns and molecular screens; for submission of such studies see Gene Expression Patterns.
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