A Multispecies Biofilm In Vitro Screening Model of Dental Caries for High-Throughput Susceptibility Testing.

Q2 Biochemistry, Genetics and Molecular Biology High-Throughput Pub Date : 2019-05-30 DOI:10.3390/ht8020014

Lara A Heersema, Hugh D C Smyth

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引用次数: 6

Abstract

There is a current need to develop and optimize new therapeutics for the treatment of dental caries, but these efforts are limited by the relatively low throughput of relevant in vitro models. The aim of this work was to bridge the 96-well microtiter plate system with a relevant multispecies dental caries model that could be reproducibly grown to allow for the high-throughput screening of anti-biofilm therapies. Various media and inoculum concentrations were assessed using metabolic activity, biomass, viability, and acidity assays to determine the optimal laboratory-controlled conditions for a multispecies biofilm composed of Streptococcus gordonii, Streptococcus mutans, and Candida albicans. The selected model encompasses several of the known fundamental characteristics of dental caries-associated biofilms. The 1:1 RPMI:TSBYE 0.6% media supported the viability and biomass production of mono- and multispecies biofilms best. Kinetic studies over 48 h in 1:1 RPMI:TSBYE 0.6% demonstrated a stable biofilm phase between 10 and 48 h for all mono- and multispecies biofilms. The 1:1:0.1 S. gordonii: S. mutans: C. albicans multispecies biofilm in 1:1 RPMI:TSBYE 0.6% is an excellent choice for a high-throughput multispecies model of dental caries. This high-throughput multispecies model can be used for screening novel therapies and for better understanding the treatment effects on biofilm interactions and stability.

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用于高通量敏感性测试的多物种生物膜体外筛选龋齿模型。

目前需要开发和优化治疗龋齿的新疗法，但这些努力受到相关体外模型相对较低吞吐量的限制。这项工作的目的是将96孔微量滴定板系统与相关的多物种龋齿模型连接起来，该模型可以重复生长，以允许高通量筛选抗生物膜疗法。使用代谢活性、生物量、活力和酸度测定来评估各种培养基和接种物浓度，以确定由戈登链球菌、变形链球菌和白色念珠菌组成的多菌种生物膜的最佳实验室控制条件。所选择的模型包含了龋齿相关生物膜的几个已知基本特征。1:1 RPMI:TSBYE 0.6%培养基对单级和多级生物膜的活力和生物量生产的支持最好。在1:1 RPMI:TSBYE 0.6%中超过48小时的动力学研究表明，所有单相和多相生物膜在10至48小时之间都有稳定的生物膜相。在1:1 RPMI:TSBYE 0.6%中的1:1:0.1戈登氏菌：变异链球菌：白色念珠菌多谱生物膜是高通量多谱龋齿模型的极好选择。该高通量多谱模型可用于筛选新疗法，并更好地了解治疗对生物膜相互作用和稳定性的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

High-Throughput Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

9 weeks

期刊介绍： High-Throughput (formerly Microarrays, ISSN 2076-3905) is a multidisciplinary peer-reviewed scientific journal that provides an advanced forum for the publication of studies reporting high-dimensional approaches and developments in Life Sciences, Chemistry and related fields. Our aim is to encourage scientists to publish their experimental and theoretical results based on high-throughput techniques as well as computational and statistical tools for data analysis and interpretation. The full experimental or methodological details must be provided so that the results can be reproduced. There is no restriction on the length of the papers. High-Throughput invites submissions covering several topics, including, but not limited to: -Microarrays -DNA Sequencing -RNA Sequencing -Protein Identification and Quantification -Cell-based Approaches -Omics Technologies -Imaging -Bioinformatics -Computational Biology/Chemistry -Statistics -Integrative Omics -Drug Discovery and Development -Microfluidics -Lab-on-a-chip -Data Mining -Databases -Multiplex Assays