Hyun-Joo Sohn, Kyung-Je Park, In-Soon Roh, Hyo-Jin Kim, Hoo-Chang Park, Hae-Eun Kang
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引用次数: 5
Abstract
Chronic wasting disease (CWD) agents are shed into biological samples, facilitating their horizontal transmission between cervid species. Once prions enter the environment, binding of PrPCWD by soil particles may maintain them near the soil surface, posing a challenge for decontamination. A 2 N sodium hydroxide (NaOH) or 2% sodium hypochlorite (NaClO) solution is traditionally recommended for prion decontamination of equipment and surfaces. Using protein misfolding cyclic amplification with beads and a bioassay with TgElk mice, we compared the effects of these disinfectants in CWD-contaminated soil for 1 or 16 h to those of controls of known infectious titres. Our results suggest that 2 N NaOH in a 1/5 farm soil volume provides a large decrease (>102-fold) in prion infectivity.
慢性消耗性疾病(CWD)病原体进入生物样本，促进它们在子宫颈物种之间的水平传播。一旦朊病毒进入环境，PrPCWD与土壤颗粒的结合可能会使它们保持在土壤表面附近，给去污带来挑战。传统上建议使用2n氢氧化钠(NaOH)或2%次氯酸钠(NaClO)溶液对设备和表面进行朊病毒净化。利用蛋白错误折叠循环扩增和TgElk小鼠的生物测定，我们比较了这些消毒剂在ccd污染土壤中1或16小时的效果与已知感染滴度的对照。我们的研究结果表明，在1/5的农田土壤体积中加入2 N NaOH可使朊病毒的传染性大幅降低(>102倍)。
期刊介绍：
Prion is the first international peer-reviewed open access journal to focus exclusively on protein folding and misfolding, protein assembly disorders, protein-based and structural inheritance. The goal is to foster communication and rapid exchange of information through timely publication of important results using traditional as well as electronic formats. The overriding criteria for publication in Prion are originality, scientific merit and general interest.
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