Molecular Detection of Leishmania major in Hemiechinus auritus: A Potential Reservoir of Zoonotic Cutaneous Leishmaniasis in Damghan, Iran.

IF 0.6 4区 医学 Q4 PARASITOLOGY Journal of Arthropod-Borne Diseases Pub Date : 2019-09-30 eCollection Date: 2019-09-01
Behrad Pourmohammadi, Sadegh Mohammadi-Azni
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Abstract

Background: Zoonotic cutaneous leishmaniasis caused by Leishmania major is endemic in 17 of 31 Iranian provinces. Various species of rodents have been introduced as the main reservoirs of the disease. This study was conducted to determine the natural infection of hedgehogs with Leishmania spp. in an endemic area of the disease, northern Iran.

Methods: Fifteen long-eared hedgehogs were captured alive during 18 months study period, from Apr 2015 to Sep 2016, in Damghan City, Semnan Province, Iran. The animals were identified using apparent characteristics and to determine the Leishmania infection, impression smears were prepared from their ear lobes, hind feet, livers, and spleens. Microscopic examination and semi-nested PCR were applied to determine the infection and to identify the parasites species respectively.

Results: All examined animals were identified as Hemiechinus auritus (Family: Erinaceidae). In microscopic examination, 8 (53.3%) samples were shown to be infected with Leishmania parasites. The higher and lower rate of the infection was observed in the ears as well as the feet and in the liver specimens, 53.3%, and 33.3% respectively. Forty percent (6/ 15) of the samples were molecularly positive and all were identified as L. major parasites. All the examined animals in autumn and 50% of them in summer were shown to be infected with Leishmania parasites.

Conclusion: This study demonstrated the natural infection of H. auritus with L. major for the first time in Damghan City and introduced these mammals as new potential reservoirs of ZCL in the study area.

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伊朗达姆甘的一个潜在人畜共患病皮肤利什曼病库中 Hemiechinus auritus 的主要利什曼原虫分子检测:伊朗达姆甘(Damghan)人畜共患皮肤利什曼病的潜在传染源。
背景:由大利什曼原虫引起的人畜共患皮肤利什曼病在伊朗 31 个省中的 17 个省流行。各种啮齿类动物是该病的主要传播源。本研究旨在确定伊朗北部该疾病流行地区刺猬自然感染利什曼原虫的情况:方法:2015 年 4 月至 2016 年 9 月的 18 个月研究期间,在伊朗塞姆南省达姆甘市活捉了 15 只长耳刺猬。根据动物表面特征对其进行鉴定,并从其耳垂、后足、肝脏和脾脏制备印模涂片,以确定利什曼原虫感染情况。显微镜检查和半嵌合 PCR 分别用于确定感染情况和寄生虫种类:结果:所有受检动物均被鉴定为赤眼蜂(Hemiechinus auritus)(科:Erinaceidae)。显微镜检查显示,8 个样本(53.3%)感染了利什曼原虫。耳朵、脚和肝脏标本的感染率较高,分别为 53.3%和 33.3%。40%(6/15)的样本分子检测呈阳性,所有样本均被鉴定为大角螨寄生虫。所有在秋季接受检查的动物和 50%在夏季接受检查的动物都感染了利什曼原虫:这项研究首次证明了达姆甘市的 H. auritus 自然感染了大叶利什曼原虫,并将这些哺乳动物作为研究地区新的潜在 ZCL 库。
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来源期刊
Journal of Arthropod-Borne Diseases
Journal of Arthropod-Borne Diseases PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY
CiteScore
2.00
自引率
11.10%
发文量
17
审稿时长
8 weeks
期刊介绍: The journal publishes original research paper, short communica­tion, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases includ­ing: ● Systematics ● Vector ecology ● Epidemiology ● Immunology ● Parasitology ● Molecular biology ● Genetics ● Population dynamics ● Toxicology ● Vector control ● Diagnosis and treatment and other related subjects.
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