Differences in Neuregulin 4 Expression in Children: Effects of Fat Depots and Obese Status.

IF 1.5 4区医学 Q4 ENDOCRINOLOGY & METABOLISM Endocrine Research Pub Date : 2020-08-01 Epub Date: 2020-01-27 DOI:10.1080/07435800.2020.1721528

Ran Wang, Wei Zhou, Xiaolei Zhu, Nan Zhou, Fan Yang, Bin Sun, Xiaonan Li

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引用次数: 4

Abstract

Purpose: To observe the expression of Nrg4, uncoupling protein-1 (UCP1), tumor necrosis factor α (TNFα), CD31, VE-cadherin/CDH5 and vascular endothelial growth factor A (VEGF-A) mRNA in abdominal subcutaneous (SC), omental (OM) adipose tissue in children with relation to anthropometric parameters. Further to verify the effect of inflammatory mediators on Nrg4 and UCP1 mRNA expression in adipocytes.

Methods: Paired SC and OM adipose tissues were obtained from 58 children. In vitro, the adipocytes isolated from primary inguinal adipose tissue of mice were treated with TNFα (50 ng/ml) for 12-48 h. mRNA levels of Nrg4, UCP1 and TNFα were determined by real-time PCR.

Results: Nrg4, UCP1, VEGF-A and CDH5 mRNA levels in SC were significantly higher than those in OM adipose tissue and the mRNA level of TNFα showed the opposite result. Moreover, Nrg4 and UCP1 mRNA in SC were significantly lower in overweight children compared to normal weight children. Nrg4 in SC and OM was negatively associated with BMISDS, WHtR. CDH55 mRNA in OM was negatively associated with WHR. VEGF-A was positively correlated with Nrg4 in SC. In vitro, Nrg4 and UCP1 mRNA levels in adipocytes were dose- and time-dependently decreased under TNFα treatment.

Conclusions: Nrg4, UCP1, VEGF-A and CDH5 mRNA expression in adipose tissues display a depot-specific pattern. Nrg4 mRNA levels in adipose tissue are decreased with obesity and associated with WAT browning and angiogenesis. TNFα may be involved in the regulation of Nrg4 level in adipose tissue, which may be one of the causes of the down-regulation of Nrg4 expression in obesity with chronic inflammatory response.

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神经调节蛋白4在儿童中的表达差异:脂肪库和肥胖状况的影响

目的:观察Nrg4、解偶联蛋白-1 (UCP1)、肿瘤坏死因子α (TNFα)、CD31、VE-cadherin/CDH5和血管内皮生长因子A (VEGF-A) mRNA在儿童腹部皮下(SC)、网膜(OM)脂肪组织中的表达与人体测量参数的关系。进一步验证炎症介质对脂肪细胞中Nrg4和UCP1 mRNA表达的影响。方法:对58例儿童的SC和OM脂肪组织进行配对。体外将小鼠原代腹股沟脂肪组织分离的脂肪细胞给予50 ng/ml TNFα处理12 ~ 48 h，采用实时荧光定量PCR法检测Nrg4、UCP1和TNFα mRNA水平。结果:SC脂肪组织中Nrg4、UCP1、VEGF-A、CDH5 mRNA水平显著高于OM脂肪组织，TNFα mRNA水平显著高于OM脂肪组织。此外，超重儿童SC中的Nrg4和UCP1 mRNA水平明显低于正常体重儿童。SC和OM中Nrg4与BMISDS、WHtR呈负相关。OM中CDH55 mRNA与WHR呈负相关。VEGF-A在SC中与Nrg4呈正相关，体外TNFα处理下，脂肪细胞中Nrg4和UCP1 mRNA水平呈剂量依赖性和时间依赖性降低。结论:Nrg4、UCP1、VEGF-A和CDH5 mRNA在脂肪组织中的表达具有仓库特异性。脂肪组织中Nrg4 mRNA水平随着肥胖而降低，并与WAT褐变和血管生成有关。TNFα可能参与了脂肪组织中Nrg4水平的调控，这可能是肥胖伴慢性炎症反应中Nrg4表达下调的原因之一。

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来源期刊

Endocrine Research 医学-内分泌学与代谢

CiteScore

4.30

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： This journal publishes original articles relating to endocrinology in the broadest context. Subjects of interest include: receptors and mechanism of action of hormones, methodological advances in the detection and measurement of hormones; structure and chemical properties of hormones. Invitations to submit Brief Reviews are issued to specific authors by the Editors.

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