Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis.

IF 3.4 2区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE BMC Complementary Medicine and Therapies Pub Date : 2020-01-23 DOI:10.1186/s12906-019-2810-3

Lei Wu, Lifen Wang, Xiangguo Tian, Junyong Zhang, Hua Feng

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引用次数: 10

Abstract

Background: Germacrone is one of the natural bioactive compounds found in Rhizoma curcuma essential oils. In this study, the potential anti-cancer effect of germacrone in gastric cancer cell line BGC823 was investigated.

Methods: The cell viability and proliferative activity were assessed, and cell cycle analysis was also performed. Hoechst 33258 and Annexin V/PI double staining was used for detection of cell apoptosis. Protein profiles of cell cycle-related and apoptosis-related proteins were assessed.

Results: MTT assay revealed that germacrone had marked cytotoxicity on BGC823 cells. Germacrone induced cell cycle arrest in the G2/M phase via remarkably decreased expression levels of cyclin B1, cdc 2 and cdc 25c. In addition, the treatment with germacrone induced caspase-3 activity and PARP cleavage. These findings demonstrated the effects of germacrone on inhibiting cell proliferation through induction of G2/M phase cell cycle arrest and promotion of cell apoptosis. It also indicated that germacrone functioned through modulations of cell cycle-associated protein expression and mitochondria-mediated apoptosis.

Conclusion: These findings will be valuable as the molecular basis for the germacrone-mediated anti-cancer effect against gastric cancer.

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德国龙通过诱导细胞周期阻滞和促进细胞凋亡对胃癌有抗癌作用。

背景:姜黄酮是姜黄精油中发现的一种天然生物活性化合物。本研究探讨了德马克酮对胃癌细胞株BGC823的潜在抗癌作用。方法:测定细胞活力和增殖活性，并进行细胞周期分析。采用Hoechst 33258和Annexin V/PI双染色法检测细胞凋亡。评估细胞周期相关蛋白和凋亡相关蛋白的蛋白谱。结果:MTT实验显示，德马克酮对BGC823细胞有明显的细胞毒性。germacron通过显著降低cyclin B1、cdc 2和cdc 25c的表达水平，诱导细胞周期阻滞在G2/M期。此外，germacone处理诱导caspase-3活性和PARP裂解。这些发现表明，germacone通过诱导G2/M期细胞周期阻滞和促进细胞凋亡来抑制细胞增殖。研究还表明，germacone通过调节细胞周期相关蛋白表达和线粒体介导的细胞凋亡发挥作用。结论:这些发现将为宏素介导的抗胃癌作用提供分子基础。

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