Effect of L-carnitine on sperm quality during liquid storage of boar semen.

IF 2.2 2区农林科学 Asian-Australasian Journal of Animal Sciences Pub Date : 2020-11-01 Epub Date: 2019-12-24 DOI:10.5713/ajas.19.0455

Kang Yang, Na Wang, Hai-Tao Guo, Jing-Ran Wang, Huan-Huan Sun, Liang-Zhen Sun, Shun-Li Yue, Jia-Bo Zhou

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引用次数: 15

Abstract

Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage.

Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooledstorage at 17°C. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated.

Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential.

Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17°C.

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左旋肉碱对猪精液液体贮存过程中精子质量的影响。

目的:研究左旋肉碱对猪精液贮存特性的影响。方法:检测精子质量，随机分为0(对照组)、12.5、25、50、100 mM左旋肉碱组。在17°C冷藏0、3、5和10天后，评估精子活力、质膜完整性和抗氧化参数(总活性氧、总抗氧化能力和丙二醛)。此外，还研究了保存的猪精子的ATP含量、线粒体活性以及精子结合和体外受精能力。结果:添加50 mM左旋肉碱能有效维持公猪精子活力和精子膜完整性等质量指标。此外，我们发现左旋肉碱对猪精子质量的积极影响主要通过提高抗氧化能力、提高ATP含量和线粒体活性来实现。有趣的是，通过评估左旋肉碱对精子生育能力和发育潜力的影响，我们发现含有左旋肉碱的扩展剂可以提高精子质量，增加精子进入透明带的数量，但不会提高体外生育能力和发育潜力。结论:在17℃液体贮存条件下，适当添加左旋肉碱可提高猪精子质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian-Australasian Journal of Animal Sciences AGRICULTURE, DAIRY & ANIMAL SCIENCE-

自引率

0.00%

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审稿时长

3 months

期刊介绍： Asian-Australasian Journal of Animal Sciences (AJAS) aims to publish original and cutting-edge research results and reviews on animal-related aspects of the life sciences. Emphasis will be placed on studies involving farm animals such as cattle, buffaloes, sheep, goats, pigs, horses, and poultry. Studies for the improvement of human health using animal models may also be publishable. AJAS will encompass all areas of animal production and fundamental aspects of animal sciences: breeding and genetics, reproduction and physiology, nutrition, meat and milk science, biotechnology, behavior, welfare, health, and livestock farming systems.