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{"title":"Mothulity Facilitates 16S/ITS Amplicon Diversity Analysis","authors":"D. Izak, A. Gromadka, S. Kaczanowski","doi":"10.1002/cpbi.94","DOIUrl":null,"url":null,"abstract":"<p>We present Mothulity—a novel interface for Mothur, a well-established tool for 16S/ITS biodiversity analysis. Although Mothur is a well-documented and virtually complete software suite, its proper execution might be challenging for first-time users, and editing the Mothur batch scripts is time consuming even for experienced users. Mothur produces little to no graphical output, leaving the generation of plots to the user. Mothulity minimizes the chance of human error through a minimalistic yet powerful interface, with most of the analysis parameters predefined or adjusted automatically. Time spent on running the analysis is drastically reduced, since Mothulity produces an HTML report with publication-quality figures. Finally, Mothulity can be conveniently used with the SLURM workload manager, and is thereby suitable for a range of computing facilities. © 2020 by John Wiley & Sons, Inc.</p><p><b>Basic Protocol 1</b>: Standard operational procedure (SOP)</p><p><b>Basic Protocol 2</b>: Generating report on pre-processed data</p>","PeriodicalId":10958,"journal":{"name":"Current protocols in bioinformatics","volume":"69 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpbi.94","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in bioinformatics","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpbi.94","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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Abstract
We present Mothulity—a novel interface for Mothur, a well-established tool for 16S/ITS biodiversity analysis. Although Mothur is a well-documented and virtually complete software suite, its proper execution might be challenging for first-time users, and editing the Mothur batch scripts is time consuming even for experienced users. Mothur produces little to no graphical output, leaving the generation of plots to the user. Mothulity minimizes the chance of human error through a minimalistic yet powerful interface, with most of the analysis parameters predefined or adjusted automatically. Time spent on running the analysis is drastically reduced, since Mothulity produces an HTML report with publication-quality figures. Finally, Mothulity can be conveniently used with the SLURM workload manager, and is thereby suitable for a range of computing facilities. © 2020 by John Wiley & Sons, Inc.
Basic Protocol 1 : Standard operational procedure (SOP)
Basic Protocol 2 : Generating report on pre-processed data
Mothulity便于16S/ITS扩增子多样性分析
我们提出了mothulity -一个新的接口，用于Mothur，一个完善的16S/ITS生物多样性分析工具。尽管motherur是一个文档完备且几乎完整的软件套件，但它的正确执行对于第一次使用的用户来说可能是一个挑战，并且编辑motherur批处理脚本即使对于有经验的用户来说也是非常耗时的。motherur几乎不产生任何图形输出，将绘图的生成留给了用户。Mothulity通过简约而强大的界面将人为错误的机会降至最低，大多数分析参数都是预定义的或自动调整的。运行分析所花费的时间大大减少了，因为Mothulity生成的HTML报告具有出版质量的数据。最后，可以方便地将Mothulity与SLURM工作负载管理器一起使用，因此适用于各种计算设施。©2020 by John Wiley &基本协议1:标准操作程序(SOP)基本协议2:对预处理数据生成报告
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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