Efficacy of intranasal and spray delivery of adjuvanted live vaccine against infectious bronchitis virus in experimentally infected poultry

Procedia in vaccinology Pub Date : 2012-01-01 DOI:10.1016/j.provac.2012.04.012

Sebastien Deville , Juliette Ben Arous , François Bertrand , Vladimir Borisov , Laurent Dupuis

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引用次数: 21

Abstract

Live vaccines are widely used in the avian industry. Such vaccines can be either injected or delivered on animal mucosa and are usually not adjuvanted. In this study we show that live vaccines efficacy can be improved by formulation with adjuvants in a model of mucosal delivery of live infectious bronchitis vaccine in chicken. Three adjuvant technologies have been tested using intranasal and spray delivery methods to poultry. Those technologies are water in oil in water emulsion, nanoparticles and polymer adjuvants. Intranasal delivery of polymer and nanoparticles adjuvanted live vaccines improved significantly the antibody titer and protection to challenge observed compared to a commercial non-adjuvanted reference. Moreover, spray delivery of the polymer adjuvanted vaccine showed a significantly higher protection compared to the non-adjuvanted reference. Our data demonstrates that the use of MontanideTM adjuvants in the formulation of live poultry vaccines for mucosal delivery can confer to vaccinated animals a significantly improved protection against pathogens.

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实验性感染家禽经鼻和喷雾注射抗传染性支气管炎病毒佐剂活疫苗的效果

活疫苗广泛应用于家禽养殖业。这种疫苗既可以注射，也可以在动物粘膜上注射，通常不需要佐剂。在这项研究中，我们表明，在鸡传染性支气管炎活疫苗的粘膜递送模型中，佐剂的配方可以提高活疫苗的效力。已经测试了三种佐剂技术，分别使用鼻内和喷雾给药方法给家禽。这些技术是水包油包水乳液、纳米颗粒和聚合物佐剂。与商业化的非佐剂活疫苗相比，经鼻给药的聚合物和纳米颗粒佐剂活疫苗显著提高了抗体滴度和对挑战的保护。此外，与未加佐剂的对照疫苗相比，聚合物佐剂疫苗的喷雾递送显示出明显更高的保护作用。我们的数据表明，在粘膜给药的活禽疫苗配方中使用MontanideTM佐剂可以使接种过疫苗的动物显著提高对病原体的保护能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Procedia in vaccinology

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