Liquid Chromatography–Mass Spectrometry (LC-MS)-Based Analysis for Lipophilic Compound Profiling in Plants

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2020-04-28 DOI:10.1002/cppb.20109

Taly Lapidot-Cohen, Leah Rosental, Yariv Brotman

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引用次数: 10

Abstract

Lipids are fascinating due to their chemical diversity, which is especially vast in the plant kingdom thanks to the high plasticity of the plant biosynthetic machinery. Lipidomic studies aim to simultaneously analyze a large number of lipid compounds of diverse classes in a given sample. The method presented here uses liquid chromatography–mass spectrometry (LC-MS)-based lipidomic profiling in a relatively fast, robust, and high-throughput manner for high-coverage quantification and annotation of lipophilic compounds. Protocols cover sample preparation, LC-MS-based measurement, and data extraction and annotation. An extensive lipid library for triacylglycerols, galactolipids, and phospholipids is provided. The extended profiling described here could be used in a range of applications and is suitable for integration with other omic datasets. © 2020 by John Wiley & Sons, Inc.

Basic Protocol 1: Sample preparation and metabolite extraction

Basic Protocol 2: Liquid chromatography–mass spectrometry (LC-MS) analysis

Basic Protocol 3: Data extraction, annotation, and quantification

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基于液相色谱-质谱(LC-MS)的植物亲脂性化合物谱分析

脂质因其化学多样性而令人着迷，由于植物生物合成机器的高度可塑性，脂质在植物界尤其广泛。脂质组学研究旨在同时分析给定样品中大量不同类别的脂质化合物。本文提出的方法采用液相色谱-质谱(LC-MS)为基础的脂质组学分析，以相对快速、稳健和高通量的方式对亲脂性化合物进行高覆盖率的定量和注释。协议涵盖样品制备，LC-MS-based测量，数据提取和注释。广泛的脂质库为三酰基甘油，半乳糖脂，磷脂提供。这里描述的扩展分析可以在一系列应用程序中使用，并且适合与其他omics数据集集成。©2020 by John Wiley &基本方案1:样品制备和代谢物提取基本方案2:液相色谱-质谱(LC-MS)分析基本方案3:数据提取，注释和定量

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Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.