{"title":"Anti-Acanthamoeba effect of potassium isostearate for use as a multipurpose solution.","authors":"Haruna Sasaki, Toshinari Koda, Hiroshi Morita","doi":"10.4265/bio.25.73","DOIUrl":null,"url":null,"abstract":"<p><p>Acanthamoeba is one of the organisms that cause corneal infection. In this study, attention was focused on potassium isostearate (iso-C18K, a branched chain fatty acid salt) for use in a multipurpose solution (MPS) against Acanthamoeba. An anti-amoebic test against Acanthamoeba castellanii ATCC 30010 (trophozoites type) was conducted. As a result, a growth reduction effect of 4 log units (99.99% suppression) was observed after incubation with 150 mM (5.0 w/v%) iso-C18K for 10 minutes. Furthermore, after the amoeba suspension was mixed with iso-C18K, disruption of cell membranes were observed, and the minimum amoebacidal concentration (MAC) at that time was 9.6 mM (0.31 w/v%). To evaluate the effectiveness as an MPS, assessment by verification tests was conducted using contact lenses. Reducing the concentration of iso-C18K caused a decrease in the number of viable cells, which was confirmed at a MAC of 1.2 mM (0.039 w/v%).</p>","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocontrol science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.4265/bio.25.73","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Acanthamoeba is one of the organisms that cause corneal infection. In this study, attention was focused on potassium isostearate (iso-C18K, a branched chain fatty acid salt) for use in a multipurpose solution (MPS) against Acanthamoeba. An anti-amoebic test against Acanthamoeba castellanii ATCC 30010 (trophozoites type) was conducted. As a result, a growth reduction effect of 4 log units (99.99% suppression) was observed after incubation with 150 mM (5.0 w/v%) iso-C18K for 10 minutes. Furthermore, after the amoeba suspension was mixed with iso-C18K, disruption of cell membranes were observed, and the minimum amoebacidal concentration (MAC) at that time was 9.6 mM (0.31 w/v%). To evaluate the effectiveness as an MPS, assessment by verification tests was conducted using contact lenses. Reducing the concentration of iso-C18K caused a decrease in the number of viable cells, which was confirmed at a MAC of 1.2 mM (0.039 w/v%).
棘阿米巴是引起角膜感染的微生物之一。在本研究中，重点研究了用于抗棘阿米巴多用途溶液(MPS)的异硬脂酸钾(iso-C18K，一种支链脂肪酸盐)。对滋养体型棘阿米巴(Acanthamoeba castellanii ATCC 30010)进行了抗阿米巴试验。结果表明，150 mM (5.0 w/v%) iso-C18K孵育10分钟后，生长抑制效果为4 log单位(99.99%)。此外，将阿米巴悬浮液与iso-C18K混合后，观察到细胞膜的破坏，此时的最小阿米巴浓度(MAC)为9.6 mM (0.31 w/v%)。为了评估MPS的有效性，使用隐形眼镜进行了验证测试。降低iso-C18K浓度导致活细胞数量减少，在MAC为1.2 mM (0.039 w/v%)时证实了这一点。
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The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.
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