Two Thalamic Regions Screened Using Laser Capture Microdissection with Whole Human Genome Microarray in Schizophrenia Postmortem Samples.

IF 3.6 Q1 PSYCHIATRY Schizophrenia Research and Treatment Pub Date : 2020-05-31 eCollection Date: 2020-01-01 DOI:10.1155/2020/5176834
Kalindi Bakshi, Eileen M Kemether
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引用次数: 2

Abstract

We used whole human genome microarray screening of highly enriched neuronal populations from two thalamic regions in postmortem samples from subjects with schizophrenia and controls to identify brain region-specific gene expression changes and possible transcriptional targets. The thalamic anterior nucleus is reciprocally connected to anterior cingulate, a schizophrenia-affected cortical region, and is also thought to be schizophrenia affected; the other thalamic region is not. Using two regions in the same subject to identify disease-relevant gene expression differences was novel and reduced intersubject heterogeneity of findings. We found gene expression differences related to miRNA-137 and other SZ-associated microRNAs, ELAVL1, BDNF, DISC-1, MECP2 and YWHAG associated findings, synapses, and receptors. Manual curation of our data may support transcription repression.

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用全基因组微阵列激光捕获显微解剖筛选精神分裂症死后样本的两个丘脑区域。
我们使用全人类基因组微阵列筛选来自精神分裂症患者和对照组死后样本的两个丘脑区域的高度富集的神经元群,以确定大脑区域特异性基因表达变化和可能的转录靶点。丘脑前核与前扣带相互连接,前扣带是受精神分裂症影响的皮质区域,也被认为是受精神分裂症影响的;另一个丘脑区域则不是。在同一受试者中使用两个区域来确定疾病相关基因表达差异是新颖的,并且减少了结果的受试者间异质性。我们发现与miRNA-137和其他sz相关的microrna、ELAVL1、BDNF、DISC-1、MECP2和YWHAG相关的发现、突触和受体相关的基因表达差异。手动管理我们的数据可能支持转录抑制。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
2
审稿时长
14 weeks
期刊介绍: Schizophrenia Research and Treatment is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to all aspects of schizophrenia.
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