Cell Deposition Microchip with Micropipette Control over Liquid Interface Motion.

Cell medicine Pub Date : 2018-05-29 eCollection Date: 2018-01-01 DOI:10.1177/2155179017733152
Daisuke Onoshima, Yuya Hattori, Hiroshi Yukawa, Kenji Ishikawa, Masaru Hori, Yoshinobu Baba
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引用次数: 1

Abstract

Positioning single cells on a solid surface is a crucial technique for understanding the cellular functions and cell-cell interactions in cell culture assays. We developed a microfluidic chip for depositing single cells in microwells using a simple micropipette operation. Cells were delivered to microwells by the meniscus motion of liquid interface. The residue deposits of cells were redistributed with air injection, and the isolated single cells were stored in microwells. Different microwell sizes and depths were studied to evaluate the trapping possibility of cells. Medium replacement and cell viability staining with the isolated single cells were achieved in microwells. The chip will serve as a tool for single-cell patterning in an easy-to-use manner.

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细胞沉积微芯片与微移液管控制的液体界面运动。
在细胞培养实验中,将单个细胞定位在固体表面是理解细胞功能和细胞间相互作用的关键技术。我们开发了一种微流控芯片,用于使用简单的微移液管操作将单细胞沉积在微孔中。细胞通过液界面的半月板运动进入微孔。通过空气注入将细胞的残留物重新分布,分离的单细胞储存在微孔中。研究了不同微孔尺寸和深度对细胞捕获的可能性。用分离的单细胞在微孔中进行培养基置换和细胞活力染色。该芯片将以易于使用的方式作为单细胞图案化的工具。
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Cell medicine
Cell medicine MEDICINE, RESEARCH & EXPERIMENTAL-
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