High day-to-day and diurnal variability of oxidative stress and inflammation biomarkers in people with type 2 diabetes mellitus and healthy individuals.

IF 5.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Redox Report Pub Date : 2020-12-01 DOI:10.1080/13510002.2020.1795587
Alistair R Mallard, Siri Marte Hollekim-Strand, Charlotte Björk Ingul, Jeff S Coombes
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引用次数: 19

Abstract

Objective: Assess the variability and differences in oxidative stress, antioxidant, and inflammatory biomarkers in people with type 2 diabetes mellitus (T2D) and healthy controls. Methods:: Ten men and women diagnosed with T2D and ten healthy matched controls (CON) were recruited. Participants had venous blood taken at six different time points on different days, three in the morning (after overnight fast) and three in the afternoon. Inflammation (IL-6, 8, 10 and TNF-α), oxidative stress/antioxidant biomarkers (F2-isoprostanes, protein carbonyls, total antioxidant capacity (TAC), glutathione peroxidase activity, IL-6, 8 & 10 and TNF-α) were assessed. Results:: Biomarker concentrations were similar between groups. There was large variability in nearly all biomarkers for both groups. For inflammatory measures, intra-individual coefficients of variation (CV) ranged from 64.0-92.1% and 100.9-259.0% for inter-individual differences. CVs for oxidative stress markers were lower (7.4-31.2% for intra-individual and 8.6-43.0% for inter-individual). TAC had the lowest intra-individual CV - 7% for T2D and 8% for CON. Protein carbonyls were more variable in the afternoon (34% CV) compared to morning (24% CV) in CON. IL-6 intra-individual CV was different between groups for afternoon measurements (93% T2D, 60% CON). Conclusion:: Oxidative stress and inflammatory biomarkers show considerable variation in both T2D and healthy populations. Trial registration: ClinicalTrials.gov identifier: NCT01206725.

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2型糖尿病患者和健康人群中氧化应激和炎症生物标志物的高日变异性
目的:评估2型糖尿病(T2D)患者和健康对照者氧化应激、抗氧化和炎症生物标志物的变异性和差异。方法:招募10名确诊为T2D的男性和女性以及10名健康匹配对照(CON)。参与者在不同天的六个不同时间点取静脉血,上午三次(禁食一夜后),下午三次。评估炎症(IL-6、8、10和TNF-α)、氧化应激/抗氧化生物标志物(f2 -异前列腺素、蛋白羰基、总抗氧化能力(TAC)、谷胱甘肽过氧化物酶活性、IL-6、8和10和TNF-α)。结果:各组间生物标志物浓度相近。两组的几乎所有生物标志物都有很大的差异。对于炎症指标,个体内变异系数(CV)的个体间差异范围为64.0-92.1%和100.9-259.0%。氧化应激标志物的CVs较低(个体内为7.4-31.2%,个体间为8.6-43.0%)。TAC的个体内CV最低,T2D为7%,CON为8%。在CON中,蛋白质羰基在下午(34% CV)比上午(24% CV)变化更大。下午测量的各组之间IL-6个体内CV不同(T2D为93%,CON为60%)。结论:氧化应激和炎症生物标志物在T2D人群和健康人群中显示出相当大的差异。试验注册:ClinicalTrials.gov标识符:NCT01206725。
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来源期刊
Redox Report
Redox Report 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
28
审稿时长
>12 weeks
期刊介绍: Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.
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