Combined therapies with exercise, ozone and mesenchymal stem cells improve the expression of HIF1 and SOX9 in the cartilage tissue of rats with knee osteoarthritis.

IF 2.2 4区 医学 Q3 PHYSIOLOGY Physiology international Pub Date : 2020-07-24 Print Date: 2020-06-01 DOI:10.1556/2060.2020.00024
Sara Asadi, Parvin Farzanegi, Mohammad Ali Azarbayjani
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引用次数: 4

Abstract

Purpose: Knee osteoarthritis (OA) is a common type of degenerative joint disease which decreases the quality of life. Sex-determining region Y box 9 (SOX9) and hypoxia-inducible factor-1 (HIF1) are considered as the key regulators of OA. We investigated the effect of combined therapies with mesenchymal stem cells (MSCs), ozone (O3) and exercise training on SOX9 and HIF1 expression in the cartilage of rats with knee OA.

Methods: Knee OA was induced by surgical method. OA rats were divided into model, MSCs, ozone, exercise, MSCs + ozone, MSCs + exercise, ozone + exercise and MSCs + ozone + exercise groups. Rats in the MSCs group received intraarticular injection of 1 × 106 cells/kg. Rats in the ozone group received O3 at the concentration of 20 μg/mL, once weekly for 3 weeks. Rats in the exercise group were trained on rodent treadmill three times per week. 48 hours after the programs, cartilage tissues were isolated and the expression of SOX9 and HIF1 was determined using Real-Time PCR.

Results: Significant differences were found in the expression of SOX9 and HIF1 between groups (P < 0.0001). Although combined therapies with exercise, MSCs and O3 significantly increased the expression of SOX9 and HIF1 in the cartilage tissue of rats with knee OA, combination of exercise with O3 was significantly more effective compared to the other combined therapies (P < 0.001).

Conclusions: Combined therapy with exercise, MSCs and O3 significantly increased the expression of SOX9 and HIF1 genes in the cartilage of rats with knee OA; however, exercise + O3 was significantly more effective.

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运动、臭氧和间充质干细胞联合治疗可改善膝骨关节炎大鼠软骨组织中HIF1和SOX9的表达。
目的:膝关节骨关节炎(OA)是一种常见的退行性关节疾病,降低了生活质量。性别决定区Y盒9 (SOX9)和缺氧诱导因子-1 (HIF1)被认为是OA的关键调控因子。我们研究了间充质干细胞(MSCs)、臭氧(O3)和运动训练联合治疗对膝关节OA大鼠软骨中SOX9和HIF1表达的影响。方法:采用手术方法诱导膝关节OA。将OA大鼠分为模型组、MSCs组、臭氧组、运动组、MSCs +臭氧组、MSCs +运动组、臭氧+运动组和MSCs +臭氧+运动组。MSCs组大鼠关节内注射1 × 106个细胞/kg。臭氧组大鼠给予浓度为20 μg/mL的O3,每周一次,连用3周。运动组的大鼠每周在啮齿动物跑步机上训练三次。程序完成48小时后,分离软骨组织,采用Real-Time PCR检测SOX9和HIF1的表达。结果:组间SOX9、HIF1表达差异有统计学意义(P < 0.0001)。虽然运动、MSCs和O3联合治疗显著增加了膝关节OA大鼠软骨组织中SOX9和HIF1的表达,但运动与O3联合治疗明显比其他联合治疗更有效(P < 0.001)。结论:MSCs和O3联合运动治疗可显著提高膝关节OA大鼠软骨组织中SOX9和HIF1基因的表达;然而,运动+ O3明显更有效。
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来源期刊
Physiology international
Physiology international Medicine-Physiology (medical)
CiteScore
3.40
自引率
0.00%
发文量
37
期刊介绍: The journal provides a forum for important new research papers written by eminent scientists on experimental medical sciences. Papers reporting on both original work and review articles in the fields of basic and clinical physiology, pathophysiology (from the subcellular organization level up to the oranizmic one), as well as related disciplines, including history of physiological sciences, are accepted.
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